Western Blot (WB)
(Western Blot analysis in T47D cell line lysates (35ug/lane) using MBS645852. This demonstrates MBS645852 detected the MAP3K12 protein (arrow).)
Mouse anti-Human MAP3K12 Monoclonal Antibody | anti-MAP3K12 antibody
MAP3K12 (MAP3K12, ZPK, Mitogen-activated protein kinase kinase kinase 12, Dual leucine zipper bearing kinase, Leucine-zipper protein kinase, MAPK-upstream kinase, Mixed lineage kinase)
Purified by ammonium sulfate precipitation.
Purified by ammonium sulfate precipitation.
Optimal dilutions to be determiend by the researcher.
Western Blot (WB)
(Western Blot analysis in T47D cell line lysates (35ug/lane) using MBS645852. This demonstrates MBS645852 detected the MAP3K12 protein (arrow).)
Western Blot (WB)
(Western Blot analysis in T47D cell line lysates (35ug/lane) using MBS645852. This demonstrates MBS645852 detected the MAP3K12 protein (arrow).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the serine/threonine protein kinase family. This kinase contains a leucine-zipper domain and is predominately expressed in neuronal cells. The phosphorylation state of this kinase in synaptic terminals was shown to be regulated by membrane depolarization via calcineurin. This kinase forms heterodimers with leucine zipper containing transcription factors, such as cAMP responsive element binding protein (CREB) and MYC, and thus may play a regulatory role in PKA or retinoic acid induced neuronal differentiation. Alternatively spliced transcript variants encoding different proteins have been described.[provided by RefSeq, Jul 2010]
Uniprot Description
Function: May be an activator of the JNK/SAPK pathway. Phosphorylates beta-casein, histone 1 and myelin basic protein in vitro.
Catalytic activity: ATP + a protein = ADP + a phosphoprotein.
Cofactor: Magnesium.
Subunit structure: Interacts with MBIP. Ref.7
Subcellular location: Cytoplasm
By similarity. Membrane; Peripheral membrane protein
By similarity.
Tissue specificity: Highly expressed in brain and kidney.
Domain: Interacts with MBIP through the leucine-zipper motif.
Post-translational modification: Autophosphorylated on Ser/Thr. Phosphorylated in cytosol under basal conditions and dephosphorylated when membrane-associated
By similarity.
Sequence similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.Contains 1 protein kinase domain.
Research Articles on MAP3K12
Similar Products
Product Notes
The MAP3K12 map3k12 (Catalog #AAA645852) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MAP3K12 (MAP3K12, ZPK, Mitogen-activated protein kinase kinase kinase 12, Dual leucine zipper bearing kinase, Leucine-zipper protein kinase, MAPK-upstream kinase, Mixed lineage kinase) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MAP3K12 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Western Blot: 1:100-1:250 Optimal dilutions to be determiend by the researcher. Researchers should empirically determine the suitability of the MAP3K12 map3k12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAP3K12, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
