Phospho-MAP3K12 Positive Control | MAP3K12 positive control

Phospho-MAP3K12 Antibody Positive Control

Cat. Num. AAA543139

• Gene Names: MAP3K12; DLK; MUK; ZPK; ZPKP1; MEKK12
• Applications: Western Blot
• Synonyms: Phospho-MAP3K12; Phospho-MAP3K12 Antibody Positive Control; DLK; Dual leucine zipper bearing kinase; Dual leucine zipper kinase DLK; Map3k12; MAPK-upstream kinase; MEKK12; mitogen-activated protein kinase kinase kinase 12; Mixed lineage kinase; MUK; Protein kinase MUK; ZPK; ZPKP1 antibody; MAP3K12 positive control

• Form/Format: Western Blot Positive Control
• Sequence Length: 567

• Applicable Applications for MAP3K12 positive control: Western Blot (WB)
• Application Notes: WB of MBS543953 with MBS543139 1 :500 antibody dilution in DiluObuffer.; Dilutions are for reference only. Applications not listed above are not necessarily precluded from working with this antibody. Investigators intending to use an application that has not been verified can request a complimentary sample.
• Immunogen: Purified Protein
• Preparation and Storage: -20 degree C for long term storage

Western Blot (WB)

Related Product Information for MAP3K12 positive control

Western Blot Positive Control Phosphorylated Mitogen-activated protein kinase kinase kinase 12 Antibody.
Overview: Mitogen-activated protein kinase kinase kinase 12 (MAP3K12) is a MAP kinase kinase kinase enzyme belonging to the serine/threonine protein kinase family (1). Located on human chromosome 12q13, it regulates the activity of various MAPKKs such as MAPKK7 and hence activating C-Jun N-terminal Kinase (JNK/SAPK). MAP3K12 associates with scaffold proteins, JIPs, which also interacts with MAPKK7 and JNKlSAPK (2). MAP3K12 protein is associated with dotted structures that are regularly located with Golgi apparatus and also along the microtubules (3). This protein contains two leucine-zipper-like motifs and interaction of MAP3K12-binding inhibitory protein with one of these motifs deactivates this protein and induces activation of SAPK/JNK (4). MAP3K12 is also implicated in regulating radial cell migration via microtubule-based events. It has been reported that ectopic expression of this protein in neuronal precursor cells allows these cells to migrate from the ventricular zone and differentiate into neural cells (3).
MAP3K12 was identified as a signaling molecule that is required for the regulation of keratinocyte terminal differentiation and cornification (5). While MAP3K12 is found in the epidermis, it is highly restricted to the neurona. Cells in the central , peripheral and autonomic nervous systems. More specifically, it is localized in the axons of these cells (6). It has been shown that over-expression of MAP3K12 in cos-1 cells impairs the radial organization of microtubules without massive depolymerization. In humans, MAP3K12 is approximately a 68kOa protein (567 amino acids). In rat and mouse this protein weighs 107kOa (888 amino acids).
The Phospho-MAP3K12 antibodies were generated using synthetic peptide corresponding to the human PMAP3K12 protein. Western blot-positive control in ready-to-use SOS-sample buffer (PC-PMAP3K12) and antigenic blocking peptide (P-PMAP3K12) are available. Antibodies can be conjugated with fluorescent probes or other secondary enzymes upon request at extra charge.

NCBI and Uniprot Product Information

• NCBI GI #: 29476775
• NCBI GeneID: 7786
• UniProt Accession #: Q12852; Q86VQ5; Q8WY25; B3KSS9; G3V1Y2
• NCBI Official Full Name: MAP3K12 protein
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 12
• NCBI Official Symbol: MAP3K12
• NCBI Official Synonym Symbols: DLK; MUK; ZPK; ZPKP1; MEKK12
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 12
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 12
• Protein Family: Mitogen-activated protein kinase kinase kinase
• UniProt Gene Name: MAP3K12
• UniProt Synonym Gene Names: ZPK; DLK; ZPK; MUK
• UniProt Entry Name: M3K12_HUMAN

NCBI Description

This gene encodes a member of the serine/threonine protein kinase family. This kinase contains a leucine-zipper domain and is predominately expressed in neuronal cells. The phosphorylation state of this kinase in synaptic terminals was shown to be regulated by membrane depolarization via calcineurin. This kinase forms heterodimers with leucine zipper containing transcription factors, such as cAMP responsive element binding protein (CREB) and MYC, and thus may play a regulatory role in PKA or retinoic acid induced neuronal differentiation. Alternatively spliced transcript variants encoding different proteins have been described.[provided by RefSeq, Jul 2010]

Uniprot Description

DLK: May be an activator of the JNK/SAPK pathway. Phosphorylates beta-casein, histone 1 and myelin basic protein in vitro. Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

Protein type: Kinase, protein; EC 2.7.11.25; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; TKL group; MLK family; LZK subfamily

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: axon; cytoplasm; cytosol; growth cone; membrane; plasma membrane

Molecular Function: ATP binding; MAP kinase kinase kinase activity; protein binding; protein homodimerization activity; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity

Biological Process: activation of MAPKK activity; histone phosphorylation; JNK cascade; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; protein amino acid autophosphorylation; protein amino acid phosphorylation