Rabbit anti-Human JPH2 Polyclonal Antibody | anti-JPH2 antibody

JPH2, CT (JPH2, JP2, Junctophilin-2, Junctophilin type 2) (Azide free) (HRP)

Cat. Num. AAA6311800

• Gene Names: JPH2; JP2; JP-2; CMH17
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: JPH2; Polyclonal Antibody; CT (JPH2; JP2; Junctophilin-2; Junctophilin type 2) (Azide free) (HRP); Junctophilin type 2; anti-JPH2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-JPH2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: JPH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 613-643 amino acids from the C-terminal region of human JPH2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(JPH2 Antibody (C-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the JPH2 antibody detected the JPH2 protein (arrow).)

Related Product Information for anti-JPH2 antibody

Junctional complexes between the plasma membrane and endoplasmic/sarcoplasmic reticulum are a common feature of all excitable cell types and mediate cross talk between cell surface and intracellular ion channels. The protein encoded by this gene is a component of junctional complexes and is composed of a C-terminal hydrophobic segment spanning the endoplasmic/sarcoplasmic reticulum membrane and a remaining cytoplasmic domain that shows specific affinity for the plasma membrane. This gene is a member of the junctophilin gene family. Alternative splicing has been observed at this locus and two variants encoding distinct isoforms are described.

Product Categories/Family for anti-JPH2 antibody

Antibodies; Connexin; Junction Proteins

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Woo, J.S., et al. Biochem. J. 427(1):125-134(2010). Yamazaki, D., et al. Pharmacol. Ther. 121(3):265-272(2009). Landstrom, A.P., et al. J. Mol. Cell. Cardiol. 42(6):1026-1035(2007). Matsushita, Y., et al. J. Hum. Genet. 52(6):543-548(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 21704281
• NCBI GeneID: 57158
• NCBI Accession #: NP_065166.2; NP_787109.2
• NCBI GenBank Nucleotide #: NM_020433.4
• UniProt Accession #: Q9BR39; O95913; Q5JY74; Q9UJN4; E1P5X1
• Molecular Weight: 13,951 Da
• NCBI Official Full Name: junctophilin-2 isoform 1
• NCBI Official Synonym Full Names: junctophilin 2
• NCBI Official Symbol: JPH2
• NCBI Official Synonym Symbols: JP2; JP-2; CMH17
• NCBI Protein Information: junctophilin-2; junctophilin type 2
• UniProt Protein Name: Junctophilin-2
• Protein Family: Junctophilin
• UniProt Gene Name: JPH2
• UniProt Synonym Gene Names: JP2; JP-2
• UniProt Entry Name: JPH2_HUMAN

NCBI Description

Junctional complexes between the plasma membrane and endoplasmic/sarcoplasmic reticulum are a common feature of all excitable cell types and mediate cross talk between cell surface and intracellular ion channels. The protein encoded by this gene is a component of junctional complexes and is composed of a C-terminal hydrophobic segment spanning the endoplasmic/sarcoplasmic reticulum membrane and a remaining cytoplasmic domain that shows specific affinity for the plasma membrane. This gene is a member of the junctophilin gene family. Alternative splicing has been observed at this locus and two variants encoding distinct isoforms are described. [provided by RefSeq, Jul 2008]

Uniprot Description

JPH2: Junctophilins contribute to the formation of junctional membrane complexes (JMCs) which link the plasma membrane with the endoplasmic or sarcoplasmic reticulum in excitable cells. Provides a structural foundation for functional cross-talk between the cell surface and intracellular calcium release channels. JPH2 is necessary for proper intracellular Ca(2+) signaling in cardiac myocytes via its involvement in ryanodine receptor-mediated calcium ion release. Contributes to the construction of skeletal muscle triad junctions. Defects in JPH2 are the cause of familial hypertrophic cardiomyopathy type 17 (CMH17). CMH17 is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Belongs to the junctophilin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Endoplasmic reticulum; Contractile

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: junctional membrane complex; plasma membrane; integral to membrane; Z disc

Molecular Function: protein binding; phosphatidylinositol-4,5-bisphosphate binding; phosphatidylinositol-3,4,5-triphosphate binding; phosphatidylserine binding; calcium-release channel activity; phosphatidylinositol-5-phosphate binding; phosphatidylinositol 3-phosphate binding

Biological Process: calcium ion homeostasis; regulation of cardiac muscle development

Disease: Cardiomyopathy, Familial Hypertrophic, 17