Rabbit anti-Human, Mouse MAP3K12 Polyclonal Antibody | anti-MAP3K12 antibody

MAP3K12, CT (Mitogen-activated Protein Kinase Kinase Kinase 12, Dual Leucine Zipper Bearing Kinase, DLK, Leucine-zipper Protein Kinase, MAPK-upstream Kinase, MUK, MEKK12, Mixed Lineage Kinase, ZPK, ZPKP1)

Cat. Num. AAA630604

• Gene Names: MAP3K12; DLK; MUK; ZPK; ZPKP1; MEKK12
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: MAP3K12; Polyclonal Antibody; CT (Mitogen-activated Protein Kinase Kinase Kinase 12; Dual Leucine Zipper Bearing Kinase; DLK; Leucine-zipper Protein Kinase; MAPK-upstream Kinase; MUK; MEKK12; Mixed Lineage Kinase; ZPK; ZPKP1); Anti -MAP3K12; anti-MAP3K12 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ZPK. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-MAP3K12 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the C-terminal region of human MAP3K12 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-ZPK C-term Pab in HL-60 cell lysate (lane A) and in mouse kidney tissue lysate (lane B). ZPK (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-MAP3K12 antibody

ZPK is a member of serine/threonine protein kinase family. This kinase contains a leucine-zipper domain, and is predominately expressed in neuronal cells. The phosphorylation state of this kinase in synaptic terminals was shown to be regulated by membrane depolarization via calcineurin. This kinase forms heterodimers with leucine zipper containing transcription factors, such as cAMP responsive element binding protein (CREB) and MYC, and thus may play a regulatory role in PKA or retinoic acid induced neuronal differentiation.

Product Categories/Family for anti-MAP3K12 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 29476775
• NCBI GeneID: 7786
• UniProt Accession #: Q12852; Q86VQ5; Q8WY25; B3KSS9; G3V1Y2
• Molecular Weight: 93,219 Da
• NCBI Official Full Name: MAP3K12 protein
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 12
• NCBI Official Symbol: MAP3K12
• NCBI Official Synonym Symbols: DLK; MUK; ZPK; ZPKP1; MEKK12
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 12; protein kinase MUK; MAPK-upstream kinase; mixed lineage kinase; leucine zipper protein kinase; dual leucine zipper kinase DLK; dual leucine zipper bearing kinase
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 12
• Protein Family: MAP3K12-binding inhibitory protein
• UniProt Gene Name: MAP3K12
• UniProt Synonym Gene Names: ZPK; DLK; ZPK; MUK
• UniProt Entry Name: M3K12_HUMAN

NCBI Description

This gene encodes a member of the serine/threonine protein kinase family. This kinase contains a leucine-zipper domain and is predominately expressed in neuronal cells. The phosphorylation state of this kinase in synaptic terminals was shown to be regulated by membrane depolarization via calcineurin. This kinase forms heterodimers with leucine zipper containing transcription factors, such as cAMP responsive element binding protein (CREB) and MYC, and thus may play a regulatory role in PKA or retinoic acid induced neuronal differentiation. Alternatively spliced transcript variants encoding different proteins have been described.[provided by RefSeq, Jul 2010]

Uniprot Description

DLK: May be an activator of the JNK/SAPK pathway. Phosphorylates beta-casein, histone 1 and myelin basic protein in vitro. Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.25; Protein kinase, TKL; Kinase, protein; TKL group; MLK family; LZK subfamily

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: growth cone; membrane; axon; cytoplasm; plasma membrane; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; protein homodimerization activity; MAP kinase kinase kinase activity; protein kinase binding; ATP binding; protein kinase activity

Biological Process: peptidyl-serine phosphorylation; activation of MAPKK activity; protein amino acid autophosphorylation; histone phosphorylation; peptidyl-threonine phosphorylation; JNK cascade; protein amino acid phosphorylation