Rabbit anti-Human Cox-17 Polyclonal Antibody | anti-Cox-17 antibody

Cox-17 (N8) Polyclonal Antibody

Cat. Num. AAA3002717

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Cox-17; Polyclonal Antibody; Cox-17 (N8) Polyclonal Antibody; Cytochrome c oxidase copper chaperone; COX17; anti-Cox-17 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: COX17 (N8) polyclonal antibody detects endogenous levels of COX17 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 63

• Applicable Applications for anti-Cox-17 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to the N-terminal of Human Cox-17.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Cox-17 (N8) pAb at 1:500 dilutionLane1:PC3 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:Panc1 whole cell lysate (40ug) Lane4:H9C2 whole cell lysate (40ug) Lane5:AML-12 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Cox-17 (N8) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-Cox-17 antibody

Cytochrome c oxidase 17 (COX17) is a nuclear gene encoding an assembly protein necessary for proper COX apoenzyme-dependent mitochondrial respiration. The mammalian COX apoenzyme is a heteromer consisting of three mitochondrial genes that encode catalytic subunits and several nuclear genes that encode structural subunits. COX17 influences the recruitment of copper to the mitochondria for incorporation into the COX apoenzyme.

NCBI and Uniprot Product Information

• NCBI GI #: 5031645
• NCBI GeneID: 10063
• NCBI Accession #: NP_005685.1
• NCBI GenBank Nucleotide #: NP_005685.1
• UniProt Accession #: Q14061
• Molecular Weight: ~ 7kDa
• NCBI Official Full Name: cytochrome c oxidase copper chaperone isoform b
• NCBI Official Synonym Full Names: cytochrome c oxidase copper chaperone COX17
• NCBI Official Symbol: COX17
• NCBI Protein Information: cytochrome c oxidase copper chaperone
• UniProt Protein Name: Cytochrome c oxidase copper chaperone
• UniProt Gene Name: COX17
• UniProt Entry Name: COX17_HUMAN

NCBI Description

Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes a protein which is not a structural subunit, but may be involved in the recruitment of copper to mitochondria for incorporation into the COX apoenzyme. This protein shares 92% amino acid sequence identity with mouse and rat Cox17 proteins. This gene is no longer considered to be a candidate gene for COX deficiency. A pseudogene COX17P has been found on chromosome 13. [provided by RefSeq, Jul 2008]

Uniprot Description

COX17: Copper chaperone for cytochrome c oxidase (COX). Binds two copper ions and deliver them to the Cu(A) site of COX. Belongs to the COX17 family.

Protein type: Mitochondrial; Energy Metabolism - oxidative phosphorylation

Chromosomal Location of Human Ortholog: 3q13.33

Cellular Component: mitochondrion; cytoplasm; mitochondrial intermembrane space

Molecular Function: copper ion binding; copper chaperone activity

Biological Process: generation of precursor metabolites and energy; heart development; brain development; copper ion transport