Mouse anti-Human MAP3K11/MLK3 Monoclonal Antibody | anti-MAP3K11/MLK3 antibody

Anti-MAP3K11/MLK3 Antibody (clone 3D11)

Cat. Num. AAA2400908

• Gene Names: MAP3K11; MLK3; PTK1; SPRK; MLK-3; MEKK11
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Purified from ascites by Protein A
• Synonyms: MAP3K11/MLK3; Monoclonal Antibody; Anti-MAP3K11/MLK3 Antibody (clone 3D11); Rabbit Polyclonal (IgG) to Human MAP3K11/MLK3; MAP3K11; Mixed lineage kinase 3; MLK3; Protein-tyrosine kinase PTK1; PTK1; SPRK; MLK-3; MEKK11; anti-MAP3K11/MLK3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a, k
• Clone Number: 3D11
• Specificity: Human MAP3K11
• Purity/Purification: Purified from ascites by Protein A
• Form/Format: PBS, pH 7.4.
• Sequence Length: 847

• Applicable Applications for anti-MAP3K11/MLK3 antibody: ELISA (EIA), Immunohistochemistry (IHC) Formalin, Western Blot (WB)
• Application Notes: IHC-Fr: 1:50
• Immunogen: MAP3K11 (NP_002410, 741 a.a. ~ 848 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C or lower.; Aliquot to avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(IHC with MAP3K11 antibody on frozen human liver.)

Immunohistochemistry (WB)

(IHC with MAP3K11 antibody on frozen human spleen.)

Western Blot (WB)

(Western blot of MAP3K11 expression in transfected 293T cell line by MAP3K11 monoclonal antibody, clone 3D11. Lane 1: MAP3K11 transfected lysate(92.7 KDa).Lane 2: Non-transfected lysate.)

ELISA (EIA)

(Detection limit for recombinant GST tagged MAP3K11 is 0.1 ng/ml as a capture antibody.)

Related Product Information for anti-MAP3K11/MLK3 antibody

Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14(p38), MAPK3 (ERK) and MAPK8 (JNK1) through phosphorylation and activation of MAP2K4/MKK4 and MAP2K7/MKK7. Plays a role inmitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization duringthe cell cycle.

Product Categories/Family for anti-MAP3K11/MLK3 antibody

Protein Kinase; MLK (MAP3K)

NCBI and Uniprot Product Information

• NCBI GI #: 4505195
• NCBI GeneID: 4296
• NCBI Accession #: NP_002410.1
• NCBI GenBank Nucleotide #: NM_002419.3
• UniProt Accession #: Q16584; Q53H00; Q59F06; Q6P2G4; B4DS76
• Molecular Weight: 65,344 Da
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase 11
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 11
• NCBI Official Symbol: MAP3K11
• NCBI Official Synonym Symbols: MLK3; PTK1; SPRK; MLK-3; MEKK11
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 11
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 11
• UniProt Gene Name: MAP3K11

NCBI Description

The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase contains a SH3 domain and a leucine zipper-basic motif. This kinase preferentially activates MAPK8/JNK kinase, and functions as a positive regulator of JNK signaling pathway. This kinase can directly phosphorylate, and activates IkappaB kinase alpha and beta, and is found to be involved in the transcription activity of NF-kappaB mediated by Rho family GTPases and CDC42. [provided by RefSeq, Jul 2008]

Uniprot Description

Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1) through phosphorylation and activation of MAP2K4/MKK4 and MAP2K7/MKK7. Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.