Rabbit KCNK13 Polyclonal Antibody | anti-KCNK13 antibody

KCNK13 Antibody

Cat. Num. AAA150682

• Gene Names: KCNK13; THIK1; THIK-1; K2p13.1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: KCNK13 Antibody is affinity chromatography purified via peptide column.
• Synonyms: KCNK13; Polyclonal Antibody; KCNK13 Antibody; THIK1; THIK-1; K2p13.1; Potassium channel subfamily K member 13; Tandem pore domain halothane-inhibited potassium channel 1; potassium channel; subfamily K; member 13; anti-KCNK13 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: KCNK13 antibody is predicted to not cross-react with other KCNK protein family members.
• Purity/Purification: KCNK13 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 408

• Applicable Applications for anti-KCNK13 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: KCNK13 antibody can be used for detection of KCNK13 by Western blot at 0.5 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: KCNK13 antibody was raised against a 15 amino acid synthetic peptide near the center of human KCNK13.
• Buffer: KCNK13 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: KCNK13 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of KCNK13 in rat brain tissue lysate with KCNK13 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.)

Immunohistochemistry (IHC)

(Immunohistochemistry of KCNK13 in human brain tissue with KCNK13 antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of KCNK13 in human brain tissue with KCNK13 antibody at 20 μg/mL.)

Related Product Information for anti-KCNK13 antibody

KCNK13 Antibody: The closely related proteins KCNK13 and KCNK12 (also known as THIK1 and 2) are the first two members of a novel two pore-forming P domains K+ channels subfamily. The pore loop domain, a highly conserved region common to all potassium channels, is involved in determining potassium ion selectivity. Members of this family are all characterized by four transmembrane domains and may function to help influence the resting membrane potential of cells. KCNK13 is expressed mainly in the brain, but is also observed in kidneys. KCNK13 has been suggested to be a candidate for the Cs+-permeable K+ channel activated by GABA(B) receptors.

NCBI and Uniprot Product Information

• NCBI GI #: 16306555
• NCBI GeneID: 56659
• NCBI Accession #: NP_071337
• NCBI GenBank Nucleotide #: NM_022054.3
• UniProt Accession #: Q9HB14; Q96E79; B5TJL8
• Molecular Weight: 45,391 Da
• NCBI Official Full Name: potassium channel subfamily K member 13
• NCBI Official Synonym Full Names: potassium channel, two pore domain subfamily K, member 13
• NCBI Official Symbol: KCNK13
• NCBI Official Synonym Symbols: THIK1; THIK-1; K2p13.1
• NCBI Protein Information: potassium channel subfamily K member 13; K2P13.1 potassium channel; potassium channel, subfamily K, member 13; tandem pore domain potassium channel THIK-1; tandem pore domain halothane-inhibited potassium channel 1
• UniProt Protein Name: Potassium channel subfamily K member 13
• Protein Family: Potassium channel subfamily
• UniProt Gene Name: KCNK13
• UniProt Synonym Gene Names: THIK-1
• UniProt Entry Name: KCNKD_HUMAN

NCBI Description

Potassium channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. This gene encodes a potassium channel containing two pore-forming domains. This protein is an open channel that can be stimulated by arachidonic acid and inhibited by the anesthetic halothane. [provided by RefSeq, Jul 2013]

Uniprot Description

KCNK13: Potassium channel displaying weak inward rectification in symmetrical K(+) solution. Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 14q32.11

Cellular Component: plasma membrane; integral to membrane

Molecular Function: potassium channel activity; voltage-gated ion channel activity

Biological Process: synaptic transmission