Rat Tumor Protein, Translationally Controlled 1 (TPT1) ELISA Kit | TPT1 elisa kit

Rat Tumor Protein, Translationally Controlled 1 (TPT1) ELISA Kit

Cat. Num. AAA2098099

• Gene Names: TPT1; HRF; p02; p23; TCTP
• Reactivity: Rat
• Synonyms: Tumor Protein; Translationally Controlled 1 (TPT1); Rat Tumor Protein; Translationally Controlled 1 (TPT1) ELISA Kit; HRF; TCTP; p02; p23; Fortilin; Translationally-Controlled Tumor Protein; Histamine-releasing factor; TPT1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Tumor Protein, Translationally Controlled 1 (TPT1).; No significant cross-reactivity or interference between Tumor Protein, Translationally Controlled 1 (TPT1) and analogues was observed.
• Sequence Length: 172

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.127ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Protein, Translationally Controlled 1 (TPT1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Protein, Translationally Controlled 1 (TPT1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TPT1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Protein, Translationally Controlled 1 (TPT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Protein, Translationally Controlled 1 (TPT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Protein, Translationally Controlled 1 (TPT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tumor Protein, Translationally Controlled 1 (TPT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for TPT1 elisa kit

Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 13097159
• NCBI GeneID: 7178
• Molecular Weight: 15,597 Da
• NCBI Official Full Name: Tumor protein, translationally-controlled 1
• NCBI Official Synonym Full Names: tumor protein, translationally-controlled 1
• NCBI Official Symbol: TPT1
• NCBI Official Synonym Symbols: HRF; p02; p23; TCTP
• NCBI Protein Information: translationally-controlled tumor protein
• UniProt Protein Name: Translationally-controlled tumor protein
• Protein Family: tRNA 2'-phosphotransferase
• UniProt Gene Name: TPT1
• UniProt Synonym Gene Names: TCTP; HRF

NCBI Description

This gene encodes a protein that is a regulator of cellular growth and proliferation. Its mRNA is highly structured and contains an oligopyrimidine tract (5'-TOP) in its 5' untranslated region that functions to repress its translation under quiescent conditions. The encoded protein is involved in a variety of cellular pathways, including apoptosis, protein synthesis and cell division. It binds to and stabilizes microtubules, and removal of this protein through phosphorylation is required for progression through mitotic and meiotic cell divisions. This gene is known to play a role in carcinogenesis, and is upregulated in some cancer cells. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017]

Uniprot Description

TPT1: a novel heat shock protein with antiapoptotic and chaperone-like activity. Its levels are highly regulated in response to various cellular stimuli and stresses. Downregulated in response to proapoptotic treatments that induce Ca(++) stress, in a PKR-dependent manner. Colocalizes with CHFR to the mitotic spindle. Appears to be involved in calcium binding and microtubule stabilization. Its exosomal secretion is induced following DNA damage. Interacts with MCL1 and STEAP3, a p53-inducible transmembrane protein that facilitates the exosomal secretion of TPT1. Secreted TPT1 acts as a histamine-releasing factor, participates in inflammatory responses by inducing the release of histamine, and may play a role in tumor reversion. May play a direct role in placental calcium transport. Phosphorylation by Plk may decrease its microtubule-stabilizing activity and promote microtubule dynamics following metaphase. Critical for peripheral T cell maintenance and TCR-mediated cell proliferation.

Protein type: Calcium-binding; Microtubule-binding

Chromosomal Location of Human Ortholog: 13q14.13

Cellular Component: cytoplasm; cytosol; extracellular space; multivesicular body; nucleus; tubulin complex

Molecular Function: calcium ion binding; microtubule binding; protein binding; RNA binding

Biological Process: calcium ion transport; cell differentiation; cellular calcium ion homeostasis; negative regulation of apoptosis; regulation of apoptosis; response to virus