Rabbit MLK3 Polyclonal Antibody | anti-MLK3 antibody

MLK3 Antibody

Cat. Num. AAA840181

• Gene Names: MAP3K11; MLK3; PTK1; SPRK; MLK-3; MEKK11
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: MLK3; Polyclonal Antibody; MLK3 Antibody; MAP3K11; SPRK; MLK-3; PTK1; MGC17114; anti-MLK3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% thimerosal.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 847

• Applicable Applications for anti-MLK3 antibody: Western Blot (WB)
• Application Notes: Western blotting (0.5-4 ug/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 92 kDa MLK3 of human, mouse, and rat origins. It does not cross-react with MLK1, MLK2 and other mixed lineage kinases.
• Immunogen: Synthetic peptide mapping to the C-terminus of human MLK3.
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: Store at -20°C. For long term storage, aliquot and freeze at -70°C. Avoid repeated freeze/defrost cycles.

Testing Data

(MBS840181Western Blot analysis of MLK3 expression with HeLa cell lysate.)

Related Product Information for anti-MLK3 antibody

Background: MLK3 (Mixed lineage kinase 3) is a serine/threonine kinase that has an amino-terminal SH3 domain followed by the kinase domain and two leucine zippers, a cdc42/Rac1 binding (CRIB) domain and several other domains/motifs at the carboxy-terminal region. MLK3 functions as a MAPKKK of the SAPK/JNK stress pathway by directly phosphorylating SEK1/MKK4 and MKK7, althomugh it is controversial whether MLK3 is involved in p38 stress pathway activation. MLK3 also functions as an IkB kinase and mediates the activation of the transcriptional factor NF-kB stimulated by CD3/CD28, smuggesting a role for MLK3 in immune and inflammatory responses.

NCBI and Uniprot Product Information

• NCBI GI #: 4505195
• NCBI GeneID: 4296
• NCBI Accession #: NP_002410.1
• NCBI GenBank Nucleotide #: NM_002419.3
• UniProt Accession #: Q16584; Q53H00; Q59F06; Q6P2G4; B4DS76
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase 11
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 11
• NCBI Official Symbol: MAP3K11
• NCBI Official Synonym Symbols: MLK3; PTK1; SPRK; MLK-3; MEKK11
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 11
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 11
• UniProt Gene Name: MAP3K11
• UniProt Entry Name: M3K11_HUMAN

NCBI Description

The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase contains a SH3 domain and a leucine zipper-basic motif. This kinase preferentially activates MAPK8/JNK kinase, and functions as a positive regulator of JNK signaling pathway. This kinase can directly phosphorylate, and activates IkappaB kinase alpha and beta, and is found to be involved in the transcription activity of NF-kappaB mediated by Rho family GTPases and CDC42. [provided by RefSeq, Jul 2008]

Uniprot Description

MLK3: a TKL kinase of the MLK family. Has an amino-terminal SH3 domain followed by the kinase domain, two leucine zipper domains, a cdc42/Rac1 binding (CRIB) domain and several other domains/motifs at the carboxy-terminal region. Phosphorylates IkappaB and activates SEK1 and MKK7.

Protein type: Protein kinase, TKL; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.25; TKL group; MLK family; MLK subfamily

Chromosomal Location of Human Ortholog: 11q13.1-q13.3

Cellular Component: microtubule; centrosome; membrane; cytoplasm

Molecular Function: identical protein binding; protein serine/threonine kinase activity; protein binding; protein homodimerization activity; JUN kinase kinase kinase activity; mitogen-activated protein kinase kinase binding; protein-tyrosine kinase activity; Rac GTPase binding; mitogen-activated protein kinase kinase kinase binding; ATP binding; protein kinase activity

Biological Process: cell death; cell proliferation; positive regulation of JNK activity; peptidyl-tyrosine phosphorylation; activation of MAPK activity; protein amino acid autophosphorylation; positive regulation of neuron apoptosis; positive regulation of JNK cascade; JNK cascade; protein amino acid phosphorylation; activation of JNK activity; activation of JNKK activity; microtubule-based process