Rabbit MLK3 Polyclonal Antibody | anti-MAP3K11 antibody

MLK3 (Mixed Lineage Kinase 3, MLK-3, Mitogen-activated Protein Kinase Kinase Kinase 11, MAP3K11, MEKK11, PTK1, Src-homology 3 Domain-containing Proline-rich Kinase, SPRK)

Cat. Num. AAA646261

• Gene Names: MAP3K11; MLK3; PTK1; SPRK; MLK-3; MEKK11
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: MLK3; Polyclonal Antibody; MLK3 (Mixed Lineage Kinase 3; MLK-3; Mitogen-activated Protein Kinase Kinase Kinase 11; MAP3K11; MEKK11; PTK1; Src-homology 3 Domain-containing Proline-rich Kinase; SPRK); Anti -MLK3 (Mixed Lineage Kinase 3; anti-MAP3K11 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MLK3 at ~92kD. Species Crossreactivity: mouse and rat.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 1% BSA, 0.02% thimerosal, 50% glycerol.

• Applicable Applications for anti-MAP3K11 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 0.5-4ug/ml
• Immunogen: Synthetic peptide corresponding to human MLK3 at C-terminus.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of MLK3 expression with HeLa cell lysate.)

Related Product Information for anti-MAP3K11 antibody

MLK3 (Mixed lineage kinase 3) is a serine/threonine kinase that has an amino-terminal SH3 domain followed by the kinase domain and two leucine zippers, a cdc42/Rac1 binding (CRIB) domain and several other domains/motifs at the carboxy-terminal region. MLK3 functions as a MAPKKK of the SAPK/JNK stress pathway by directly phosphorylating SEK1/MKK4 and MKK7, although it is controversial whether MLK3 is involved in p38 stress pathway activation. MLK3 also functions as an IkB kinase and mediates the activation of the transcriptional factor NF-kB stimulated by CD3/CD28, suggesting a role for MLK3 in immune and inflammatory responses.

Product Categories/Family for anti-MAP3K11 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 4505195
• NCBI GeneID: 4296
• NCBI Accession #: NP_002410.1
• NCBI GenBank Nucleotide #: NM_002419.3
• UniProt Accession #: Q16584; Q53H00; Q59F06; Q6P2G4
• Molecular Weight: 92,688 Da
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase 11
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 11
• NCBI Official Symbol: MAP3K11
• NCBI Official Synonym Symbols: MLK3; PTK1; SPRK; MLK-3; MEKK11
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 11; mixed lineage kinase 3; protein-tyrosine kinase PTK1; SH3 domain-containing proline-rich kinase; src-homology 3 domain-containing proline-rich kinase
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 11
• Protein Family: Mitogen-activated protein kinase kinase kinase
• UniProt Gene Name: MAP3K11
• UniProt Synonym Gene Names: MLK3; PTK1; SPRK
• UniProt Entry Name: M3K11_HUMAN

NCBI Description

The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase contains a SH3 domain and a leucine zipper-basic motif. This kinase preferentially activates MAPK8/JNK kinase, and functions as a positive regulator of JNK signaling pathway. This kinase can directly phosphorylate, and activates IkappaB kinase alpha and beta, and is found to be involved in the transcription activity of NF-kappaB mediated by Rho family GTPases and CDC42. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1) through phosphorylation and activation of MAP2K4/MKK4 and MAP2K7/MKK7. Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle. Ref.1 Ref.5 Ref.9 Ref.10

Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Ref.1

Cofactor: Magnesium

By similarity.

Enzyme regulation: Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation. Ref.6 Ref.7

Subunit structure: Homodimer; undergoes dimerization during activation. Interacts with MAP2K4/MKK4. Interacts with MAP2K7/MKK7

By similarity. Ref.5 Ref.6

Subcellular location: Cytoplasm › cytoskeleton › microtubule organizing center › centrosome. Note: Location is cell cycle dependent. Ref.9

Tissue specificity: Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and adult lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain. Ref.1 Ref.2

Post-translational modification: Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-277 is likely to be the main autophosphorylation site. Phosphorylation of Ser-555 and Ser-556 is induced by CDC42.

Sequence similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.Contains 1 protein kinase domain.Contains 1 SH3 domain.

Sequence caution: The sequence BAD92892.1 differs from that shown. Reason: Erroneous initiation.