Western Blot (WB)
(Western Blot Analysis:|Non-stimulated PC-12 (Lane 1) or NGF stimulated PC-12 (Lane 2) cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with MBS606026 (1:5000 dilution, Lane 1) or total anti-MAP Kinase.|Proteins were visualized using a goat antirabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. |Arrows indicate phospho-MAP Kinase 1 (Erk1) (~44kDa) and phospho-MAP Kinase 2 (Erk2) (~42kDa).)
Rabbit anti-Human MAP Kinase p44/42 Monoclonal Antibody | anti-MAPK1 antibody
MAP Kinase p44/42, phosphorylated (Thr185/Tyr187) (MAPK3/MAPK1, Mitogen-activated Protein Kinase 1, MAP Kinase 1, MAPK 1, ERT1, Extracellular Signal-regulated Kinase 2, ERK-2, MAP Kinase Isoform p42, p42-MAPK, Mitogen-activated Protein Kinase 2, MAP Kinas
Reactivity
Human
Applications
Western Blot
Purity
SupernatantSupernatant
Synonyms
MAP Kinase p44/42; Monoclonal Antibody; phosphorylated (Thr185/Tyr187) (MAPK3/MAPK1; Mitogen-activated Protein Kinase 1; MAP Kinase 1; MAPK 1; ERT1; Extracellular Signal-regulated Kinase 2; ERK-2; MAP Kinase Isoform p42; p42-MAPK; Mitogen-activated Protein Kinase 2; MAP Kinas; Anti -MAP Kinase p44/42; anti-MAPK1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Clone Number
10F648
Specificity
Recognizes human phosphorylated MAP Kinase 1 and 2 (Erk 1&2). Sequence homology: rat.
Purity/Purification
Supernatant
Supernatant
Supernatant
Form/Format
Supplied as a liquid, 0.05% sodium azide.
Applicable Applications for anti-MAPK1 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot and Multiplexing.,
Dilution: Western Blot: 1:5000
Dilution: Western Blot: 1:5000
Immunogen
Synthetic peptide corresponding to amino acids surrounding the pTEpY motif of human phospho-MAP Kinase 1/2 (Erk1/2), in which the Thr and Tyr residues are phosphorylated (KLH). The immunizing sequence is highly conserved among species.
Positive Control
RIPA cell lysate from NGF stimulated PC-12 cells.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot Analysis:|Non-stimulated PC-12 (Lane 1) or NGF stimulated PC-12 (Lane 2) cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with MBS606026 (1:5000 dilution, Lane 1) or total anti-MAP Kinase.|Proteins were visualized using a goat antirabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. |Arrows indicate phospho-MAP Kinase 1 (Erk1) (~44kDa) and phospho-MAP Kinase 2 (Erk2) (~42kDa).)
Western Blot (WB)
(Western Blot Analysis:|Non-stimulated PC-12 (Lane 1) or NGF stimulated PC-12 (Lane 2) cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with MBS606026 (1:5000 dilution, Lane 1) or total anti-MAP Kinase.|Proteins were visualized using a goat antirabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. |Arrows indicate phospho-MAP Kinase 1 (Erk1) (~44kDa) and phospho-MAP Kinase 2 (Erk2) (~42kDa).)
Related Product Information for anti-MAPK1 antibody
Erk (Extracellular signal-Related Kinase) is a family of two, highly homologous proteins denoted as Erk1 (p44, MAPK3) and Erk2 (p42, MAPK1) that both function in the same pathway. The two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase. The Erk pathway is considered the classical, canonical MAPK (Mitogen-Activated Protein Kinase) signaling pathway. It is an evolutionarily conserved pathway that controls and is a critical regulator the growth and survival through the promotion of cell proliferation and the prevention of apoptosis. Erk is involved in the control of many fundamental cellular processes including cell proliferation, survival, differentiation, apoptosis, motility and metabolism. Erk is activated by growth factor stimulation of receptor tyrosine kinases (RTKs) and/or integrin stimulation. This activates the Ras-Raf-MEK,-Erk pathway that results in MEK, a dual kinase (a Ser/Thr and a Tyr kinase), phosphorylation and activation of ERK1/2 (p44/044) on the TxY motif (Thr202/Tyr204 and Thr185/Tyr187 for Erk1 and Erk2, respectively).
Product Categories/Family for anti-MAPK1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
mitogen-activated protein kinase 1
NCBI Official Synonym Full Names
mitogen-activated protein kinase 1
NCBI Official Symbol
MAPK1
NCBI Protein Information
mitogen-activated protein kinase 1
Research Articles on MAPK1
Similar Products
Product Notes
The MAPK1 (Catalog #AAA606026) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MAP Kinase p44/42, phosphorylated (Thr185/Tyr187) (MAPK3/MAPK1, Mitogen-activated Protein Kinase 1, MAP Kinase 1, MAPK 1, ERT1, Extracellular Signal-regulated Kinase 2, ERK-2, MAP Kinase Isoform p42, p42-MAPK, Mitogen-activated Protein Kinase 2, MAP Kinas reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MAP Kinase p44/42 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot and Multiplexing., Dilution: Western Blot: 1:5000. Researchers should empirically determine the suitability of the MAPK1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAP Kinase p44/42, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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