Rabbit MAP Kinase p44/42 Monoclonal Antibody | anti-MAPK1 antibody

MAP Kinase p44/42 (MAPK3/MAPK1, Mitogen-activated Protein Kinase 1, MAP Kinase 1, MAPK 1, ERT1, Extracellular Signal-regulated Kinase 2, ERK-2, MAP Kinase Isoform p42, p42-MAPK, Mitogen-activated Protein Kinase 2, MAP Kinase 2, MAPK 2, ERK2, PRKM1, PRKM2

Cat. Num. AAA605910

• Reactivity: Bovine, C elegans, Hamster, Human, Monkey, Mouse, Porcine, Rat0
• Applications: Flow Cytometry, Functional Assay
• Purity: Supernatant; Supernatant
• Synonyms: MAP Kinase p44/42; Monoclonal Antibody; MAP Kinase p44/42 (MAPK3/MAPK1; Mitogen-activated Protein Kinase 1; MAP Kinase 1; MAPK 1; ERT1; Extracellular Signal-regulated Kinase 2; ERK-2; MAP Kinase Isoform p42; p42-MAPK; Mitogen-activated Protein Kinase 2; MAP Kinase 2; MAPK 2; ERK2; PRKM1; PRKM2; Anti -MAP Kinase p44/42 (MAPK3/MAPK1; anti-MAPK1 antibody

• Host: Rabbit
• Reactivity: Bovine, C elegans, Hamster, Human, Monkey, Mouse, Porcine, Rat0
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 10E37
• Specificity: Recognizes human p44/42 MAPK. Species Crossreactivity: mouse, rat, monkey, mink, porcine, hamster, bovine, zebrafish, S. cerevisiae, D. melanogaster and C. elegans.
• Purity/Purification: Supernatant; Supernatant
• Form/Format: Supplied as a liquid in PBS (pH 7.2), less than 0.1% sodium azide, 2mg/ml BSA. Labeled with Alexa Fluor® 647.

• Applicable Applications for anti-MAPK1 antibody: Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Flow Cytometry.; Dilution: Flow Cytometry: 1:50.
• Immunogen: A synthetic peptide (KLH-coupled) corresponding to residues near the carboxy terminus of rat p44 MAP kinase.
• Preparation and Storage: May be stored at 4 degree C. For long-term storage, aliquot and store at 4 degree C. Do not freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.; Note: We do not recommended adding glycerol as a cryopreservative.

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Jurkat cells using MBS605910 (green) compared to mAb IgG Isotype Control (Alexa Fluor® 647 Conjugate) (blue).)

Related Product Information for anti-MAPK1 antibody

Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3) and is an important target in the identification and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors such as U0126 and PD98059.

Product Categories/Family for anti-MAPK1 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 45383812
• NCBI GeneID: 373953
• NCBI Accession #: NP_989481.1
• NCBI GenBank Nucleotide #: NM_204150.1
• NCBI Official Full Name: mitogen-activated protein kinase 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 1
• NCBI Official Symbol: MAPK1
• NCBI Protein Information: mitogen-activated protein kinase 1