Western Blot (WB)
(Western blot analysis of p44/42 MAP Kinase (Tyr204) in NIH-3T3 cells.)
Rabbit MAP Kinase p44/42 Polyclonal Antibody | anti-MAPK1 antibody
MAP Kinase p44/42, phosphorylated (Tyr204) (MAPK3/MAPK1, Mitogen-activated Protein Kinase 1, MAP Kinase 1, MAPK 1, ERT1, Extracellular Signal-regulated Kinase 2, ERK-2, MAP Kinase Isoform p42, p42-MAPK, Mitogen-activated Protein Kinase 2, MAP Kinase 2, MA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
MAP Kinase p44/42; Polyclonal Antibody; phosphorylated (Tyr204) (MAPK3/MAPK1; Mitogen-activated Protein Kinase 1; MAP Kinase 1; MAPK 1; ERT1; Extracellular Signal-regulated Kinase 2; ERK-2; MAP Kinase Isoform p42; p42-MAPK; Mitogen-activated Protein Kinase 2; MAP Kinase 2; MA; Anti -MAP Kinase p44/42; anti-MAPK1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human MAP Kinase p44/42, phosphorylated at Tyr204. Species Crossreactivity: mouse and rat.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Applicable Applications for anti-MAPK1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot and Immunohistochemistry.
Dilution: Western Blot: 1:500-1:1000
Immunohistochemistry (paraffin): 1:50-1:100
Dilution: Western Blot: 1:500-1:1000
Immunohistochemistry (paraffin): 1:50-1:100
Immunogen
Synthetic phosphopeptide corresponding to human p44/42 MAP Kinase around the phosphorylation site of tyrosine 204 (T-E-YP-V-A).
Positive Control
NIH-3T3 cells
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of p44/42 MAP Kinase (Tyr204) in NIH-3T3 cells.)
Western Blot (WB)
(Western blot analysis of p44/42 MAP Kinase (Tyr204) in NIH-3T3 cells.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of p44/42 MAP Kinase (Tyr204) in paraffin-embedded human breast carcinoma tissue.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of p44/42 MAP Kinase (Tyr204) in paraffin-embedded human breast carcinoma tissue.)
Related Product Information for anti-MAPK1 antibody
Human p44/42 MAP Kinase belongs to the family of signal transduction intermediates leading to cell proliferation or differentiation, known as MAPKs (mitogen-activated protein kinases). It plays an important role in the signal transduction pathways that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals.Activation of the protein requires phosphorylation of both tyrosine and threonine residues by upstream dual-specific kinases. Active MAPKs are responsible for the phosphorylation of a variety of effector proteins, including several transcription factors.
Product Categories/Family for anti-MAPK1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
mitogen-activated protein kinase 1
NCBI Official Synonym Full Names
mitogen-activated protein kinase 1
NCBI Official Symbol
MAPK1
NCBI Protein Information
mitogen-activated protein kinase 1
Research Articles on MAPK1
Similar Products
Product Notes
The MAPK1 (Catalog #AAA6001191) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MAP Kinase p44/42, phosphorylated (Tyr204) (MAPK3/MAPK1, Mitogen-activated Protein Kinase 1, MAP Kinase 1, MAPK 1, ERT1, Extracellular Signal-regulated Kinase 2, ERK-2, MAP Kinase Isoform p42, p42-MAPK, Mitogen-activated Protein Kinase 2, MAP Kinase 2, MA reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MAP Kinase p44/42 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot and Immunohistochemistry. Dilution: Western Blot: 1:500-1:1000 Immunohistochemistry (paraffin): 1:50-1:100. Researchers should empirically determine the suitability of the MAPK1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAP Kinase p44/42, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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