Western Blot (WB)
(Western blot analysis of extracts from NIH/3T3 cells untreated or treated with Phorbol-12-Myristate-13-Acetate (PMA), using Phospho-p44/42 MAPK (Thr 202/Tyr204) (IHC Preferred) T8260-08 (upper) or p44/42 MAPK (lower). This assay serves as a control for the efficacy of the PMA treatment.)
MAP Kinase p44/42 Immunoglobulin
MAP Kinase p44/42, phosphorylated (Thr202, Tyr204), Immunohistochemistry Controls (MAPK3/MAPK1, Mitogen-activated Protein Kinase 1, MAP Kinase 1, MAPK 1, ERT1, Extracellular Signal-regulated Kinase 2, ERK-2, MAP Kinase Isoform p42, p42-MAPK, Mitogen-activ
Synonyms
MAP Kinase p44/42; phosphorylated (Thr202; Tyr204); Immunohistochemistry Controls (MAPK3/MAPK1; Mitogen-activated Protein Kinase 1; MAP Kinase 1; MAPK 1; ERT1; Extracellular Signal-regulated Kinase 2; ERK-2; MAP Kinase Isoform p42; p42-MAPK; Mitogen-activ; MAP Kinase p44/42 immunoglobulin
Application Notes
These slides are intended for use in immunohistochemical assays. Please see the Companion Products for a list of prodcucts that can be used with these slides.
Preparation and Storage
Store at 4 degree C. Optimal staining is achieved if slides are stained following our standard IHC protocols and are used within 8 weeks of assay date; however, signals may persist beyond two months.
Western Blot (WB)
(Western blot analysis of extracts from NIH/3T3 cells untreated or treated with Phorbol-12-Myristate-13-Acetate (PMA), using Phospho-p44/42 MAPK (Thr 202/Tyr204) (IHC Preferred) T8260-08 (upper) or p44/42 MAPK (lower). This assay serves as a control for the efficacy of the PMA treatment.)
Western Blot (WB)
(Western blot analysis of extracts from NIH/3T3 cells untreated or treated with Phorbol-12-Myristate-13-Acetate (PMA), using Phospho-p44/42 MAPK (Thr 202/Tyr204) (IHC Preferred) T8260-08 (upper) or p44/42 MAPK (lower). This assay serves as a control for the efficacy of the PMA treatment.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded NIH/3T3 cells, untreated (left) or treated with Phorbol-12-Myristate-13-Acetate (PMA) (right), using Phospho-p44/42MAPK (Thr202 /Tyr204) (IHC Preferred))
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded NIH/3T3 cells, untreated (left) or treated with Phorbol-12-Myristate-13-Acetate (PMA) (right), using Phospho-p44/42MAPK (Thr202 /Tyr204) (IHC Preferred))
Related Product Information for MAP Kinase p44/42 immunoglobulin
p44/42 MAPK (Erk1 and Erk2), SAPK/JNK and p38 MAPK function in protein kinase cascades that play a critical role in the regulation of cell growth, differentiation and control of cellular responses to cytokines and stress.p44/42 MAPK (Erk1 and Erk2) is activated by growth and neurotrophic factors. Activation occurs through phosphorylation of threonine and tyrosine (202 and 204 of human MAP kinase [Erk1]) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK).SAPK/JNK and p38 MAPK are activated by inflammatory cytokines and a wide variety of cellular stresses. Activation of SAPK/JNK occurs via phosphorylation at Thr183 and Tyr185 by the dual specificity enzyme SEK/MKK4. Both MKK3 and SEK phosphorylate p38 MAPK on tyrosine and threonine at the sequence T*GY*, resulting in p38 activation.
Sections are cut freshly upon ordering.
Each control slide contains formalin fixed, paraffin-embedded NIH/3T3 cells, both untreated and treated with Phorbol-13-Myristate-12-Acetate (PMA), that serve as a control for Phospho-p44/42 MAPK (Thr202/Tyr204) immunostaining. PMA induces phosphorylation of p44/42 MAPK. Western Blot analysis was performed on extracts derived from the same cells to verify the efficacy of the the PMA treatment.
Sections are cut freshly upon ordering.
Each control slide contains formalin fixed, paraffin-embedded NIH/3T3 cells, both untreated and treated with Phorbol-13-Myristate-12-Acetate (PMA), that serve as a control for Phospho-p44/42 MAPK (Thr202/Tyr204) immunostaining. PMA induces phosphorylation of p44/42 MAPK. Western Blot analysis was performed on extracts derived from the same cells to verify the efficacy of the the PMA treatment.
Product Categories/Family for MAP Kinase p44/42 immunoglobulin
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Product Notes
The MAP Kinase p44/42 (Catalog #AAA652636) is an Immunoglobulin and is intended for research purposes only. The product is available for immediate purchase. These slides are intended for use in immunohistochemical assays. Please see the Companion Products for a list of prodcucts that can be used with these slides. Researchers should empirically determine the suitability of the MAP Kinase p44/42 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAP Kinase p44/42, Immunoglobulin" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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