Rabbit Cyclin D1 Polyclonal Antibody | anti-D1 antibody

Cyclin D1 (D86) Polyclonal Antibody

Cat. Num. AAA3002612

• Gene Names: CCND1; BCL1; PRAD1; U21B31; D11S287E
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Cyclin D1; Polyclonal Antibody; Cyclin D1 (D86) Polyclonal Antibody; G1/S-specific cyclin-D1; B-cell lymphoma 1 protein; BCL-1; BCL-1 oncogene; PRAD1 oncogene; CCND1; BCL1; PRAD1; anti-D1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Cyclin D1 (D86) polyclonal antibody detects endogenous levels of Cyclin D1 protein
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 295

• Applicable Applications for anti-D1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 55-100 of Human Cyclin D1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Cyclin D1 (D86) polyclonal antibody at 1:500 dilutionLane1:Hela whole cell lysate (40ug) Lane2:RAW264. 7 whole cell lysate (40ug) Lane3:PC12 whole cell lysate (40ug) Lane4:NIH-3T3 whole cell lysate (40ug) Lane5:HEK293T whole cell lysate (40ug))

Related Product Information for anti-D1 antibody

Considerable effort directed towards the identification of G1 cyclins has led to the isolation of cyclin D, cyclin C and cyclin E. Of these, cyclin D corresponds to a putative human oncogene, designated PRAD1, which maps at the site of the Bcl1 rearrangement in certain lymphomas and leukemias. Two additional human type D cyclins, as well as their mouse homologs, have been identified. Evidence has established that members of the cyclin D family function to regulate phosphorylation of the retinoblastoma gene product, thereby activating E2F transcription factors.

NCBI and Uniprot Product Information

• NCBI GI #: 16950655
• NCBI GeneID: 595
• NCBI Accession #: NP_444284.1
• NCBI GenBank Nucleotide #: NP_444284.1
• UniProt Accession #: P24385
• Molecular Weight: ~ 36kDa
• NCBI Official Full Name: G1/S-specific cyclin-D1
• NCBI Official Synonym Full Names: cyclin D1
• NCBI Official Symbol: CCND1
• NCBI Official Synonym Symbols: BCL1; PRAD1; U21B31; D11S287E
• NCBI Protein Information: G1/S-specific cyclin-D1
• UniProt Protein Name: G1/S-specific cyclin-D1
• Protein Family: Chromosomal protein
• UniProt Gene Name: CCND1
• UniProt Synonym Gene Names: BCL1; PRAD1; BCL-1
• UniProt Entry Name: CCND1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of human cancers. [provided by RefSeq, Dec 2019]

Uniprot Description

CCND1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb.

Protein type: Cell cycle regulation; Nuclear receptor co-regulator; Activator; Oncoprotein

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: nucleoplasm; tight junction; membrane; transcriptional repressor complex; cyclin-dependent protein kinase holoenzyme complex; intracellular; nucleus; cytosol

Molecular Function: protein binding; enzyme binding; histone deacetylase binding; protein complex binding; cyclin-dependent protein kinase regulator activity; transcription corepressor activity; protein kinase binding; transcription factor binding; protein kinase activity

Biological Process: lactation; G1 DNA damage checkpoint; fat cell differentiation; establishment and/or maintenance of chromatin architecture; re-entry into mitotic cell cycle; positive regulation of cyclin-dependent protein kinase activity; negative regulation of Wnt receptor signaling pathway; positive regulation of mammary gland epithelial cell proliferation; negative regulation of epithelial cell differentiation; negative regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin; protein amino acid phosphorylation; response to magnesium ion; response to vitamin E; Leydig cell differentiation; response to iron ion; response to X-ray; response to corticosterone stimulus; response to drug; Notch signaling pathway; organ regeneration; transcription, DNA-dependent; unfolded protein response; mammary gland epithelial cell proliferation; response to organic nitrogen; liver development; response to ethanol; cell division; response to estrogen stimulus; mitotic cell cycle; positive regulation of protein amino acid phosphorylation; response to calcium ion; response to DNA damage stimulus; G1/S transition of mitotic cell cycle

Disease: Von Hippel-lindau Syndrome; Myeloma, Multiple