Testing Data
(Immunoperoxidase staining of a mouse lymph node with Rat anti Mouse CD301 antibody, clone ER-MP23 (MBS216035) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (MBS235195). Low power)
Rat CD301 Monoclonal Antibody | anti-CD301 antibody
RAT ANTI MOUSE CD301
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
Synonyms
CD301; Monoclonal Antibody; RAT ANTI MOUSE CD301; anti-CD301 antibody
Host
Rat
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
ER-MP23
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Applicable Applications for anti-CD301 antibody
Immunohistology Frozen, Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunohistology Paraffin*
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/100; Maximum Dilution: 1/200
Immunohistology - Paraffin: Application Note: Heat mediated antigen retrieval is required prior to staining paraffin-embedded sections with this antibody. MyBioSource recommend citrate buffer, pH6.0, for this purpose.
Flow Cytometry: Minimum Dilution: 1/100; Maximum Dilution: 1/200
Immunohistology - Paraffin: Application Note: Heat mediated antigen retrieval is required prior to staining paraffin-embedded sections with this antibody. MyBioSource recommend citrate buffer, pH6.0, for this purpose.
Perservative Stabilisers
0.09% Sodium Azide
Preparation
Preparation
Immunogen
Balb/c macrophage precursor cell hybrids
Fusion Partners
Fusion Partners
Target Species
Mouse
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Testing Data
(Immunoperoxidase staining of a mouse lymph node with Rat anti Mouse CD301 antibody, clone ER-MP23 (MBS216035) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (MBS235195). Low power)
Testing Data
(Immunoperoxidase staining of a mouse lymph node with Rat anti Mouse CD301 antibody, clone ER-MP23 (MBS216035) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (MBS235195). Low power)
Testing Data
(Immunoperoxidase staining of a mouse lymph node with Rat anti Mouse CD301 antibody, clone ER-MP23 (MBS216035) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (MBS235195). Medium power)
Testing Data
(Immunoperoxidase staining of a mouse lymph node with Rat anti Mouse CD301 antibody, clone ER-MP23 (MBS216035) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (MBS235195). Medium power)
Testing Data
(Immunoperoxidase staining of a mouse lymph node with Rat anti Mouse CD301 antibody, clone ER-MP23 (MBS216035) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (MBS235195). High power)
Testing Data
(Immunoperoxidase staining of a mouse lymph node with Rat anti Mouse CD301 antibody, clone ER-MP23 (MBS216035) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (MBS235195). High power)
Related Product Information for anti-CD301 antibody
Rat anti Mouse CD301 antibody, clone ER-MP23 recognizes murine CD301, a 38 kD cell surface protein that is otherwise known as macrophage galactose N-acetylgalactosamine lectin (MGL) or dendritic cell asialoglycoprotein (DC-ASGPR). In mice, CD301 is predominantly expressed on mature macrophages found associated with a wide range of connective tissues including macrophages in the dermis and the pancreas. Clone ER-MP23 also detects a population of dendritic cells in lymphoid tissue, which are probably recent immigrants from peripheral connective tissue sites. Expression of CD301 is induced by alternative (i.e. IL-4/IL-13 mediated) activation of macrophages and dendritic cells, but not all CD301 positive cells are necessarily IL-4/IL-13 stimulated. Clone ER-MP23 is reported to block the function of mouse CD301. The antibody binds both MGL1 and MGL2 homologues.
Similar Products
Product Notes
The CD301 (Catalog #AAA216041) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD301 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunohistology Paraffin*. Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Minimum Dilution: 1/100; Maximum Dilution: 1/200 Immunohistology - Paraffin: Application Note: Heat mediated antigen retrieval is required prior to staining paraffin-embedded sections with this antibody. MyBioSource recommend citrate buffer, pH6.0, for this purpose. Researchers should empirically determine the suitability of the CD301 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD301, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
