Immunofluorescence (IF)
(Immunofluorescence analysis of MCF-7 cell using 212253y. Blue: DAPI for nuclear staining.)
Rabbit NeuN Polyclonal Antibody | anti-NeuN antibody
NeuN (Neuron Specific Nuclear Protein, FLJ56884, FLJ58356, FOX3, HRNBP3, RBFOX3, NEUN, FOX-3) (Biotin)
Gene Names
RBFOX3; FOX3; NEUN; FOX-3; HRNBP3
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Western Blot, Immunohistochemistry
Purity
Purified by affinity chromatography.
Synonyms
NeuN; Polyclonal Antibody; NeuN (Neuron Specific Nuclear Protein; FLJ56884; FLJ58356; FOX3; HRNBP3; RBFOX3; NEUN; FOX-3) (Biotin); anti-NeuN antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human NeuN. Species Crossreactivity: mouse and rat
Purity/Purification
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Concentration
~1mg/ml (varies by lot)
Applicable Applications for anti-NeuN antibody
Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes
IF: 1:50-1:200
WB: 1:500-1:2000
IHC-P: 1:50-1:200
Applications are based on unconjugated antibody.
WB: 1:500-1:2000
IHC-P: 1:50-1:200
Applications are based on unconjugated antibody.
Immunogen
Recombinant protein corresponding to human NeuN.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunofluorescence (IF)
(Immunofluorescence analysis of MCF-7 cell using 212253y. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of MCF-7 cell using 212253y. Blue: DAPI for nuclear staining.)
Product Categories/Family for anti-NeuN antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Calculated MW: 33kDa/35kDa
Observed MW: 50kDa
Observed MW: 50kDa
NCBI Official Full Name
RNA binding protein fox-1 homolog 3
NCBI Official Synonym Full Names
RNA binding protein, fox-1 homolog (C. elegans) 3
NCBI Official Symbol
RBFOX3
NCBI Official Synonym Symbols
FOX3; NEUN; FOX-3; HRNBP3
NCBI Protein Information
RNA binding protein fox-1 homolog 3; fox-1 homolog C; neuronal nuclei; hexaribonucleotide binding protein 3
UniProt Protein Name
RNA binding protein fox-1 homolog 3
UniProt Gene Name
RBFOX3
UniProt Entry Name
RFOX3_HUMAN
Uniprot Description
NeuN: RNA-binding protein that regulates alternative splicing events.
Protein type: RNA splicing; RNA processing; DNA-binding
Chromosomal Location of Human Ortholog: 17q25.3
Cellular Component: cytoplasm; perikaryon; nucleus
Molecular Function: DNA binding; RNA binding; nucleotide binding
Biological Process: RNA splicing; mRNA processing; regulation of alternative nuclear mRNA splicing, via spliceosome
Research Articles on NeuN
Similar Products
Product Notes
The NeuN rbfox3 (Catalog #AAA6428808) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NeuN (Neuron Specific Nuclear Protein, FLJ56884, FLJ58356, FOX3, HRNBP3, RBFOX3, NEUN, FOX-3) (Biotin) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NeuN can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Paraffin. IF: 1:50-1:200 WB: 1:500-1:2000 IHC-P: 1:50-1:200 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the NeuN rbfox3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NeuN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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