Rat CD13 Monoclonal Antibody | anti-CD13 antibody

RAT ANTI MOUSE CD13

Cat. Num. AAA212549

• Gene Names: Anpep; Apn; AP-M; AP-N; Cd13; P150
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Synonyms: CD13; Monoclonal Antibody; RAT ANTI MOUSE CD13; anti-CD13 antibody

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: R3-63
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 966

• Applicable Applications for anti-CD13 antibody: Immunohistology Frozen, Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunohistology Paraffin*
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. ; Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100; Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. See Bertilaccio et al. for details.
• Perservative Stabilisers: 0.09% Sodium Azide; Preparation
• Immunogen: Mouse intestinal APN; Fusion Partners
• Target Species: Mouse
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data

(Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD13 antibody, clone R3-63, green in A and Rat anti Mouse CD8, clone YTS105.18 , red in B. Cis the merged image with nuclei counterstained blue using DAPI. High power)

Testing Data

(Staining of mouse peripheral blood mononuclear cells with Rat anti Mouse CD13: Alexa Fluor 647 (MBS212549A647))

Testing Data

(Immunoperoxidase staining of mouse lymph node cryosection eith Rat anti Mouse antibody clone R3-63 followed by horseradish peroxidase Goat anti Rat IgG antibody (MBS235195). High power)

Testing Data

(Immunoperoxidase staining of mouse lymph node cryosection eith Rat anti Mouse antibody clone R3-63 followed by horseradish peroxidase Goat anti Rat IgG antibody (MBS235195). Low power)

Testing Data

(Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD13 antibody, clone R3-63, green in A and Rat anti Mouse CD8, clone YTS105.18 , red in B. Cis the merged image with nuclei counterstained blue using DAPI. Low power)

Testing Data

(Staining of mouse peripheral blood mononuclear cells with Rat anti Mouse CD13: Alexa Fluor 488 (MBS212549A488))

Testing Data

(Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD13 antibody, clone R3-63, green in A and Rat anti Mouse CD8, clone YTS105.18 , red in B. Cis the merged image with nuclei counterstained blue using DAPI. Medium power)

Testing Data

(Immunoperoxidase staining of mouse lymph node cryosection eith Rat anti Mouse antibody clone R3-63 followed by horseradish peroxidase Goat anti Rat IgG antibody (MBS235195). Medium power)

Related Product Information for anti-CD13 antibody

Rat anti Mouse CD13 antibody, clone R3-63 recognizes mouse aminopeptidase N (APN), a cell surface protein homologous with human CD13. In the mouse, CD13 is a non-covalently linked homodimer of approximately 150kDa subunits expressed by a variety of cells including monocytes, macrophages, dendritic cell and veiled cells. Rat anti Mouse CD13 antibody, clone R3-63 has been reported to block mouse APN enzyme activity (Kamoun et al. 2009).

NCBI and Uniprot Product Information

• NCBI GI #: 225637487
• NCBI GeneID: 16790
• NCBI Accession #: NP_032512.2
• NCBI GenBank Nucleotide #: NM_008486.2
• UniProt Accession #: P97449; Q91YH8; Q99K63
• Molecular Weight: 109,651 Da
• NCBI Official Full Name: aminopeptidase N
• NCBI Official Synonym Full Names: alanyl (membrane) aminopeptidase
• NCBI Official Symbol: Anpep
• NCBI Official Synonym Symbols: Apn; AP-M; AP-N; Cd13; P150
• NCBI Protein Information: aminopeptidase N; alanyl aminopeptidase; aminopeptidase M; aminopeptidase N/CD13; membrane protein p161; microsomal aminopeptidase
• UniProt Protein Name: Aminopeptidase N
• UniProt Gene Name: Anpep
• UniProt Synonym Gene Names: Lap-1; Lap1; AP-N; mAPN; AP-M
• UniProt Entry Name: AMPN_MOUSE

Uniprot Description

CD13: Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection. Belongs to the peptidase M1 family.

Protein type: Other Amino Acids Metabolism - glutathione; Protease; Receptor, misc.; Membrane protein, integral; EC 3.4.11.2

Cellular Component: extracellular space; membrane; lysosomal membrane; integral to membrane; ER-Golgi intermediate compartment; vesicle membrane; external side of plasma membrane

Molecular Function: peptidase activity; zinc ion binding; metallopeptidase activity; hydrolase activity; metal ion binding; aminopeptidase activity; peptide binding

Biological Process: multicellular organismal development; aromatic compound metabolic process; angiogenesis; cell differentiation; proteolysis