WB (Western Blot)
(Detection of human SKA3 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-SKA3 antibody AAA212550 (lot AAA212550-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human SKA3 Polyclonal Antibody | anti-SKA3 antibody
Rabbit anti-SKA3 Antibody, Affinity Purified
Gene Names
SKA3; RAMA1; C13orf3
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
SKA3, Antibody; Rabbit anti-SKA3 Antibody, Affinity Purified; C13orf3; RAMA1; spindle and kinetochore-associated protein 3; Spindle and kinetochore-associated protein 3; spindle and kinetochore associated complex subunit 3; anti-SKA3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-SKA3 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 362 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human SKA3 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-SKA3 antibody AAA212550 (lot AAA212550-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human SKA3 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-SKA3 antibody AAA212550 (lot AAA212550-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human SKA3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-SKA3 antibody AAA212550 (lot AAA212550-1) used for IP at 6 ug/mg lysate. SKA3 was also immunoprecipitated by rabbit anti-SKA3 antibody For blotting immunoprecipitated SKA3, AAA212550 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human SKA3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-SKA3 antibody AAA212550 (lot AAA212550-1) used for IP at 6 ug/mg lysate. SKA3 was also immunoprecipitated by rabbit anti-SKA3 antibody For blotting immunoprecipitated SKA3, AAA212550 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-SKA3 antibody
Spindle and kinetochore associated complex subunit (SKA3) is a component of the spindle and kinetochore-associated protein complex that regulates microtubule attachment to the kinetochores during mitosis. SKA3 localizes to the outer kinetechore and may be required for normal chromosome segregation and cell division [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q8IX90].
Product Categories/Family for anti-SKA3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43,978 Da
NCBI Official Full Name
spindle and kinetochore-associated protein 3 isoform 1
NCBI Official Synonym Full Names
spindle and kinetochore associated complex subunit 3
NCBI Official Symbol
SKA3
NCBI Official Synonym Symbols
RAMA1; C13orf3
NCBI Protein Information
spindle and kinetochore-associated protein 3
UniProt Protein Name
Spindle and kinetochore-associated protein 3
UniProt Gene Name
SKA3
UniProt Synonym Gene Names
C13orf3; RAMA1
UniProt Entry Name
SKA3_HUMAN
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Product Notes
The SKA3 ska3 (Catalog #AAA212550) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-SKA3 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SKA3 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the SKA3 ska3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SKA3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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