Immunofluorescence (IF)
(Immunofluorescence staining of fixed K562 cells with anti-CD15 antibody BRA-4F1 (MBS488724) Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of BRA-4F1 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
Rabbit anti-Human CD15 Monoclonal Antibody | anti-CD15 antibody
Anti-CD15 [BRA-4F1]
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Immunofluorescence, Immunohistochemistry
Purity
Purified antibody. Protein A affinity purified
Synonyms
CD15; Monoclonal Antibody; Anti-CD15 [BRA-4F1]; sLeX; Lewis X antigen; Leu-M1; Sialyl-Lewis X; SSEA-1; sialyl LeX; anti-CD15 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG, kappa
Clone Number
BRA-4F1
Specificity
This antibody is specific for CD15 (Appelmelk. et al, 1996, pmid:8675304). It also shows crossreactivity with the CD15 antigen from other species.
Purity/Purification
Purified antibody. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CD15 antibody
Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes
This antibody has been used in flow cytometry (Appelmelk. et al, 1996). The antibody has been shown to be usefull in immunohistochemistry (Appelmelk. et al, 1996). The antibody is utilized in immunopercipitation (Akin. et al, 2000). Immunofluorescence is a proven application of this antibody (Vilella. et al, 2003). CD15 is expressed in Reed-Sternberg cells, which are a sign of Hodgkin's lymphoma, and thus the antibody can be used in immunohistochemistry to detect this kind of cancer (Trillo-Tinoco. et al, 2019). Finally, BRA-4F1 can be used in fertility research as the CD15 antigen is a molecule that is nessecary for a sperm cell to Recognise and fertilize an egg cell.
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgM format, for improved compatibility with existing reagents, assays and techniques.
PMID
8675304
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Immunofluorescence (IF)
(Immunofluorescence staining of fixed K562 cells with anti-CD15 antibody BRA-4F1 (MBS488724) Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of BRA-4F1 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of fixed K562 cells with anti-CD15 antibody BRA-4F1 (MBS488724) Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of BRA-4F1 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
Related Product Information for anti-CD15 antibody
Recombinant monoclonal antibody to CD15. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma BRA-4F1. Nordic MUBio
This antibody was raised by immunzing mice with human CD15.
This antibody was raised by immunzing mice with human CD15.
Product Categories/Family for anti-CD15 antibody
References
Appelmelk. et al Potential Role of Molecular Mimicry between Helicobacter pylori Lipopolysaccharide and Host Lewis Blood Group Antigens in Autoimmunity INFECTION AND IMMUNITY, June 1996, p. 2031-2040
Note on publication: The article describes the binding of the antibody to CD15 compared to another anti-CD15 antibody.
Note on publication: The article describes the binding of the antibody to CD15 compared to another anti-CD15 antibody.
Similar Products
Product Notes
The CD15 (Catalog #AAA488725) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD15 [BRA-4F1] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD15 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC). This antibody has been used in flow cytometry (Appelmelk. et al, 1996). The antibody has been shown to be usefull in immunohistochemistry (Appelmelk. et al, 1996). The antibody is utilized in immunopercipitation (Akin. et al, 2000). Immunofluorescence is a proven application of this antibody (Vilella. et al, 2003). CD15 is expressed in Reed-Sternberg cells, which are a sign of Hodgkin's lymphoma, and thus the antibody can be used in immunohistochemistry to detect this kind of cancer (Trillo-Tinoco. et al, 2019). Finally, BRA-4F1 can be used in fertility research as the CD15 antigen is a molecule that is nessecary for a sperm cell to Recognise and fertilize an egg cell. Researchers should empirically determine the suitability of the CD15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD15, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
