Mouse Interferon Gamma (IFNg) Monoclonal Antibody | anti-IFNg antibody

Monoclonal Antibody to Interferon Gamma (IFNg)

Cat. Num. AAA2090619

• Gene Names: Ifng; Ifg; IFN-g
• Reactivity: Mus musculus (Mouse)
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
• Purity: Protein A + Protein G affinity chromatography
• Synonyms: Interferon Gamma (IFNg); Monoclonal Antibody; Monoclonal Antibody to Interferon Gamma (IFNg); IFN-G; IFG; IFI; INFr; IFN; Immune Interferon; anti-IFNg antibody

• Host: Mouse
• Reactivity: Mus musculus (Mouse)
• Clonality: Monoclonal
• Isotype: IgG2b Kappa
• Clone Number: C3
• Purity/Purification: Protein A + Protein G affinity chromatography
• Form/Format: Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-IFNg antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
• Application Notes: Western blotting: 0.5-2ug/mL;; Immunohistochemistry: 5-20ug/mL;; Immunocytochemistry: 5-20ug/mL;; Optimal working dilutions must be determined by end user.
• Source: Monoclonal antibody preparation
• Traits: Liquid
• Preparation and Storage: Storage:; Avoid repeated freeze/thaw cycles.; Store at 4 degree C for frequent use.; Aliquot and store at -20 degree C for 24 months.; ; Stability Test:; thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Recombinant IFNg, Mouse.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Liver Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Kidney Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Testis Tissue.)

NCBI and Uniprot Product Information

• NCBI GI #: 33468859
• NCBI GeneID: 15978
• NCBI Accession #: NP_032363.1
• NCBI GenBank Nucleotide #: NM_008337.4
• UniProt Accession #: P01580; Q542B8
• NCBI Official Full Name: interferon gamma
• NCBI Official Synonym Full Names: interferon gamma
• NCBI Official Symbol: Ifng
• NCBI Official Synonym Symbols: Ifg; IFN-g
• NCBI Protein Information: interferon gamma
• UniProt Protein Name: Interferon gamma
• Protein Family: Interferon
• UniProt Gene Name: Ifng
• UniProt Synonym Gene Names: IFN-gamma

NCBI Description

This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mice deficient in this gene have increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015]

Uniprot Description

IFNG: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. Homodimer. Released primarily from activated T lymphocytes. Belongs to the type II (or gamma) interferon family.

Protein type: Cytokine; Membrane protein, integral; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10 D2|10 66.75 cM

Cellular Component: cytoplasm; external side of plasma membrane; extracellular region; extracellular space; neuron projection; perikaryon

Molecular Function: cytokine activity; interferon-gamma receptor binding; protein binding

Biological Process: adaptive immune response; antigen processing and presentation; apoptosis; CD8-positive, alpha-beta T cell differentiation during immune response; cell cycle arrest; cellular response to interleukin-18; defense response to bacterium; defense response to protozoan; defense response to virus; endoplasmic reticulum unfolded protein response; humoral immune response; immune response; inflammatory cell apoptotic process; negative regulation of cell proliferation; negative regulation of epithelial cell differentiation; negative regulation of fibroblast proliferation; negative regulation of gene expression; negative regulation of glomerular mesangial cell proliferation; negative regulation of interleukin-17 production; negative regulation of myelination; negative regulation of smooth muscle cell proliferation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; neutrophil apoptosis; neutrophil chemotaxis; positive regulation of apoptosis; positive regulation of autophagy; positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation during immune response; positive regulation of cell adhesion; positive regulation of cell proliferation; positive regulation of chemokine biosynthetic process; positive regulation of core promoter binding; positive regulation of epithelial cell migration; positive regulation of gene expression; positive regulation of interleukin-1 beta secretion; positive regulation of interleukin-12 biosynthetic process; positive regulation of interleukin-12 production; positive regulation of interleukin-23 production; positive regulation of interleukin-6 biosynthetic process; positive regulation of isotype switching to IgG isotypes; positive regulation of killing of cells of another organism; positive regulation of membrane protein ectodomain proteolysis; positive regulation of MHC class II biosynthetic process; positive regulation of neuron differentiation; positive regulation of nitric oxide biosynthetic process; positive regulation of osteoclast differentiation; positive regulation of peptidyl-serine phosphorylation of STAT protein; positive regulation of protein complex assembly; positive regulation of protein deacetylation; positive regulation of protein import into nucleus, translocation; positive regulation of protein phosphorylation; positive regulation of protein serine/threonine kinase activity; positive regulation of smooth muscle cell apoptotic process; positive regulation of synaptic transmission, cholinergic; positive regulation of T cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; positive regulation of tumor necrosis factor (ligand) superfamily member 11 production; positive regulation of tumor necrosis factor production; positive regulation of tyrosine phosphorylation of STAT protein; protein import into nucleus, translocation; regulation of defense response to virus by host; regulation of growth; regulation of hepatocyte proliferation; regulation of immune response; regulation of insulin secretion; regulation of neuronal action potential; regulation of protein ADP-ribosylation; regulation of the force of heart contraction; regulation of transcription, DNA-templated; response to virus; sensory perception of mechanical stimulus; T cell receptor signaling pathway