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Western Blot (WB) (Anti- Integrin alpha 4 Picoband antibody, MBS178144, Western blottingAll lanes: Anti Integrin alpha 4 (MBS178144) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Rat Kidney Tissue Lysate at 50ugLane 3: JURKAT Whole Cell Lysate at 40ugLane 4: 22RV1 Whole Cell Lysate at 40ugLane 5: HEPG2 Whole Cell Lysate at 40ugLane 6: SMMC Whole Cell Lysate at 40ugPredicted bind size: 115KDObserved bind size: 220KD )

anti-Human, Rat Integrin alpha 4 Polyclonal Antibody | anti-ITGA4 antibody

Anti-Integrin alpha 4 Antibody

Gene Names
ITGA4; IA4; CD49D
Reactivity
Human, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen Affinity Purified
Synonyms
Integrin alpha 4; Polyclonal Antibody; Anti-Integrin alpha 4 Antibody; Integrin alpha-4; 269C wild type; Alpha 4 subunit of VLA 4 receptor; Antigen CD49D; alpha 4 subunit of VLA 4 receptor; CD 49d; CD49 antigen like family member D; CD49 antigen-like family member D; CD49d; CD49d antigen; CDw49d; IA4; Integrin alpha 4 subunit; Integrin alpha IV; Integrin alpha-IV; Integrin; alpha 4 (antigen CD49D; alpha 4 subunit of VLA 4 receptor); ITA4_HUMAN; ITGA 4; ITGA4; LPAM23; MGC90518; OTTHUMP00000205320; Very Late Activation Antigen 4; Very Late Activation Protein 4 Receptor Alpha 4 Subunit; Very late activation protein 4 receptor; alpha 4 subunit; VLA 4; VLA 4 subunit alpha; VLA-4 subunit alpha; VLA4; integrin; alpha 4 subunit of VLA-4 receptor); anti-ITGA4 antibody
Ordering
For Research Use Only!
Reactivity
Human, Rat
Clonality
Polyclonal
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
1032
Applicable Applications for anti-ITGA4 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes
Western Blot Concentration: 0.1-0.5ug/ml
Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Integrin alpha 4 (999-1028aa MWKAGFFKRQYKSILQEENRRDSWSYINS K), different from the related mouse sequence by one amino acid.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- Integrin alpha 4 Picoband antibody, MBS178144, Western blottingAll lanes: Anti Integrin alpha 4 (MBS178144) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Rat Kidney Tissue Lysate at 50ugLane 3: JURKAT Whole Cell Lysate at 40ugLane 4: 22RV1 Whole Cell Lysate at 40ugLane 5: HEPG2 Whole Cell Lysate at 40ugLane 6: SMMC Whole Cell Lysate at 40ugPredicted bind size: 115KDObserved bind size: 220KD )

Western Blot (WB) (Anti- Integrin alpha 4 Picoband antibody, MBS178144, Western blottingAll lanes: Anti Integrin alpha 4 (MBS178144) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Rat Kidney Tissue Lysate at 50ugLane 3: JURKAT Whole Cell Lysate at 40ugLane 4: 22RV1 Whole Cell Lysate at 40ugLane 5: HEPG2 Whole Cell Lysate at 40ugLane 6: SMMC Whole Cell Lysate at 40ugPredicted bind size: 115KDObserved bind size: 220KD )

Immunohistochemistry (IHC)

(Anti- Integrin alpha 4 Picoband antibody, MBS178144, IHC(P)IHC(P): Human Mammary Cancer Tissue )

Immunohistochemistry (IHC) (Anti- Integrin alpha 4 Picoband antibody, MBS178144, IHC(P)IHC(P): Human Mammary Cancer Tissue )
Related Product Information for anti-ITGA4 antibody
Description: Rabbit IgG polyclonal antibody for Integrin alpha-4(ITGA4) detection. Tested with WB, IHC-P in Human;Rat.

Background: ITGA4/Integrin alpha4 is an integrin alpha subunit. It makes up half of the alpha4beta1 lymphocyte homing receptor. The product of this gene belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes an alpha 4 chain. Unlike other integrin alpha chains, alpha 4 neither contains an I-domain, nor undergoes disulfide-linked cleavage. Alpha 4 chain associates with either beta 1 chain or beta 7 chain. ITGA4 is also mapped to 2q31-q32 by fluorescence in situ hybridization.
References
1. Jaspers, M., Zhang, Z., Marynen, P., Vekemans, S., Aly, M. S., Cuppens, H., Hillicker, C., Cassiman, J.-J.Localization of the genes encoding the alpha-2 and alpha-4 subunits of the human VLA-receptors to chromosome 5q23-31 and 2q31-32 respectively. (Abstract)Cytogenet. Cell Genet. 58: 1870 only, 1991. 2. Miller, D. H., Khan, O. A., Sheremata, W. A., Blumhardt, L. D., Rice, G. P. A., Libonati, M. A., Willmer-Hulme, A. J., Dalton, C. M., Miszkiel, K. A., O'Connor, P. W.A controlled trial of natalizumab for relapsing multiple sclerosis.New Eng. J. Med. 348: 15-23, 2003.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
21,536 Da
NCBI Official Full Name
integrin alpha-4 isoform 1 preproprotein
NCBI Official Synonym Full Names
integrin subunit alpha 4
NCBI Official Symbol
ITGA4
NCBI Official Synonym Symbols
IA4; CD49D
NCBI Protein Information
integrin alpha-4
UniProt Protein Name
Integrin alpha-4
Protein Family
UniProt Gene Name
ITGA4
UniProt Synonym Gene Names
CD49D
UniProt Entry Name
ITA4_HUMAN

NCBI Description

The gene encodes a member of the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 4 subunit. This subunit associates with a beta 1 or beta 7 subunit to form an integrin that may play a role in cell motility and migration. This integrin is a therapeutic target for the treatment of multiple sclerosis, Crohn's disease and inflammatory bowel disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]

Uniprot Description

ITGA4: Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha- 4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha- 4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. Heterodimer of an alpha and a beta subunit. The alpha subunit can sometimes be cleaved into two non-covalently associated fragments. Alpha-4 associates with either beta-1 or beta-7. Alpha-4 interacts with PXN, LPXN, and TGFB1I1/HIC5. Interacts with CSPG4 through CSPG4 chondroitin sulfate glycosaminoglycan. Belongs to the integrin alpha chain family.

Protein type: Receptor, misc.; Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q31.3

Cellular Component: cell surface; focal adhesion; membrane; plasma membrane

Molecular Function: C-X3-C chemokine binding; cell adhesion molecule binding; coreceptor activity; fibronectin binding; metal ion binding; protein binding

Biological Process: B cell differentiation; cell-matrix adhesion; diapedesis; extracellular matrix organization and biogenesis; heterotypic cell-cell adhesion; integrin-mediated signaling pathway; leukocyte adhesion; leukocyte migration; leukocyte tethering or rolling; receptor clustering; regulation of immune response

Research Articles on ITGA4

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Product Notes

The ITGA4 itga4 (Catalog #AAA178144) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Integrin alpha 4 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Integrin alpha 4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Western Blot Concentration: 0.1-0.5ug/ml Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml. Researchers should empirically determine the suitability of the ITGA4 itga4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Integrin alpha 4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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