Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Vinculin (VCL) Standard Curve.)
Mouse Vinculin (VCL) ELISA Kit | VCL elisa kit
Mouse Vinculin (VCL) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Vinculin (VCL) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Vinculin (VCL) Standard Curve.)
Background: In mammalian cells, vinculin is a membrane-cytoskeletal protein in focal adhesion plaques that is involved in linkage of integrin adhesion molecules to the actin cytoskeleton. Its sequence is 20-30% homologous to alpha-catenin, which serves a similar function. Vinculin is a 117 kDa cytoskeletal protein with 1066 amino acids. The protein contains an acidic N-terminal domain and a basic C-terminal domain separated by a proline-rich middle segment. Vinculin consists of a globular head domain that contains binding sites for talin and alpha-actinin as well as a tyrosine phosphorylation site, while the tail region contains binding sites for F-actin, paxillin and lipids.
NCBI and Uniprot Product Information
Uniprot Description
Vinculin: Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. Exhibits self-association properties. Interacts with NRAP and SORBS1. Interacts with TLN1. Interacts with SYNM. Interacts with CTNNB1 and this interaction is necessary for its localization to the cell-cell junctions and for its function in regulating cell surface expression of E-cadherin. Metavinculin is muscle-specific. Belongs to the vinculin/alpha-catenin family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal; Cell adhesion
Cellular Component: actin cytoskeleton; actin filament; adherens junction; cell junction; cell-cell adherens junction; costamere; cytoplasm; cytoskeleton; fascia adherens; focal adhesion; intercellular junction; lipid raft; membrane; plasma membrane; protein complex; sarcolemma; stress fiber; Z disc
Molecular Function: actin binding; actin filament binding; alpha-catenin binding; protein binding; Rho GTPase binding; structural molecule activity; ubiquitin protein ligase binding
Biological Process: apical junction assembly; axon extension; cell adhesion; lamellipodium biogenesis; morphogenesis of an epithelium; regulation of cell migration
Research Articles on VCL
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Product Notes
The Mouse VCL vcl (Catalog #AAA9716637) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716637 ELISA Kit recognizes Mouse VCL. It is sometimes possible for the material contained within the vial of "Vinculin (VCL), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
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