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Western Blot (WB) (Western blot analysis of HSP90alpha mAb-HRP: Lane 1: HSP90 Native Protein, Lane 2: HSP90alpha Recombinant Protein, Lane 3: HeLa Cell Lysate (Heat Shocked).)

Rat HSP90alpha Monoclonal Antibody | anti-HSP90alpha antibody

HSP90alpha monoclonal antibody (9D2) (HRP conjugate)

Gene Names
HSP90AA1; EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; LAP-2; HSP89A; HSP90A; HSP90N; Hsp103; HSPCAL1; HSPCAL4; HEL-S-65p
Reactivity
Human, Chicken
Applications
Western Blot
Purity
Protein G affinity purified.
Synonyms
HSP90alpha; Monoclonal Antibody; HSP90alpha monoclonal antibody (9D2) (HRP conjugate); HSP86; Heat shock protein 90alpha; mAb (9D2) (HRP conjugate); anti-HSP90alpha antibody
Ordering
For Research Use Only!
Host
Rat
Reactivity
Human, Chicken
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
9D2
Specificity
Recognizes human and chicken HSP90alpha. Detects a band of ~90kDa by Western blot.
Purity/Purification
Protein G affinity purified.
Form/Format
Liquid. In PBS, pH 7.2, containing 0.09% sodium azide diluted in proprietary stabilizer solution.
Sequence Length
732
Applicable Applications for anti-HSP90alpha antibody
Western Blot (WB)
Application Notes
Western Blot (1:2000-1:50, 000).
Detects a band of ~90kDa by Western blot.
Immunogen
Purified Hsp90 isolated from human therapeutic orchiectomy specimens.
Preparation and Storage
Long Term: -20 degree C
Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of HSP90alpha mAb-HRP: Lane 1: HSP90 Native Protein, Lane 2: HSP90alpha Recombinant Protein, Lane 3: HeLa Cell Lysate (Heat Shocked).)

Western Blot (WB) (Western blot analysis of HSP90alpha mAb-HRP: Lane 1: HSP90 Native Protein, Lane 2: HSP90alpha Recombinant Protein, Lane 3: HeLa Cell Lysate (Heat Shocked).)
Related Product Information for anti-HSP90alpha antibody
The 90 kDa molecular chaperone family includes 90 kDa heat shock protein Hsp90 and 94 kDa glucose-regulated protein grp94, both major molecular chaperones of the cytosol and the endoplasmic reticulum. Mammalian cells contain isoforms Hsp90 alpha and Hsp90 beta, encoded by separate genes. The amino acid sequences of human and yeast Hsp90 alpha are 85% and 90% homologous to those of Hsp90 beta, respectively. All known members of the Hsp90 protein family are highly conserved, especially in the N -terminal and C-terminal regions containing independent chaperone sites with different substrate specificity. These ubiquitous and highly conserved proteins account for 1-2% of all cellular proteins in most cells. Hsp90 functions as part of the cell
Product Categories/Family for anti-HSP90alpha antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
98,161 Da
NCBI Official Full Name
Heat shock protein HSP 90-alpha
NCBI Official Synonym Full Names
heat shock protein 90 alpha family class A member 1
NCBI Official Symbol
HSP90AA1
NCBI Official Synonym Symbols
EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; LAP-2; HSP89A; HSP90A; HSP90N; Hsp103; HSPCAL1; HSPCAL4; HEL-S-65p
NCBI Protein Information
heat shock protein HSP 90-alpha
UniProt Protein Name
Heat shock protein HSP 90-alpha
UniProt Gene Name
HSP90AA1
UniProt Synonym Gene Names
HSP90A; HSPC1; HSPCA; HSP 86; HSP86; LAP-2; LPS-associated protein 2

NCBI Description

The protein encoded by this gene is an inducible molecular chaperone that functions as a homodimer. The encoded protein aids in the proper folding of specific target proteins by use of an ATPase activity that is modulated by co-chaperones. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity which is essential for its chaperone activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes (PubMed:11274138, PubMed:11276205, PubMed:15577939, PubMed:15937123, PubMed:27353360).

Research Articles on HSP90alpha

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Product Notes

The HSP90alpha hsp90aa1 (Catalog #AAA565741) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The HSP90alpha monoclonal antibody (9D2) (HRP conjugate) reacts with Human, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's HSP90alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot (1:2000-1:50, 000). Detects a band of ~90kDa by Western blot. Researchers should empirically determine the suitability of the HSP90alpha hsp90aa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSP90alpha, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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