Rabbit Heat Shock Protein 90 Alpha Polyclonal Antibody | anti-HSP90AA1 antibody

Rabbit anti Heat Shock Protein 90 Alpha

Cat. Num. AAA225116

• Gene Names: HSP90AA1; EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; LAP-2; HSP89A; HSP90A; HSP90N; Hsp103; HSPCAL1; HSPCAL4; HEL-S-65p
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified whole serum by affinity chromatography.
• Synonyms: Heat Shock Protein 90 Alpha; Polyclonal Antibody; Rabbit anti Heat Shock Protein 90 Alpha; Heat Shock Protein 90 Alpha Antibody; anti-HSP90AA1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified whole serum by affinity chromatography.
• Form/Format: Liquid in phosphate buffered saline with 0.02% sodium azide, 50% glycerol.
• Sequence Length: 732

• Applicable Applications for anti-HSP90AA1 antibody: Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IF: 1:20-1:50; IHC-Paraffin: 1:50-1:100; WB: 1:500-1:2000
• Immunogen: Recombinant human heat shock protein 90 alpha
• Preperation: Antiserum to heat shock protein 90 alpha was raised by repeated immunization of rabbits with highly purified antigen.
• Preparation and Storage: Store at -20 degree C.; Storage in frost-free freezers is not recommended. This product should be stored undiluted.; Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch

Western Blot (WB)

(Western blot analysis of cell and tissue lysates using Rabbit anti heat shock protein 90 alpha antibody)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human prostate using Rabbit anti heat shock protein 90 alpha antibody at a dilution of 1/100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human lung using Rabbit anti heat shock protein 90 alpha antibody at a dilution of 1/100)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cells using Rabbit anti heat shock protein 90 alpha antibody)

Related Product Information for anti-HSP90AA1 antibody

Rabbit anti heat shock protein 90 alpha antibody recognizes heat shock protein 90 alpha also known as HSP90AA1 or LAP2. Heat shock protein 90 alpha is an isoform of the highly conserved heat shock protein 90. Heat shock protein 90 alpha expression is induced under conditions of cellular stress where upon it acts as a molecular chaperone.

NCBI and Uniprot Product Information

• NCBI GI #: 92090606
• NCBI GeneID: 3320
• UniProt Accession #: P07900; Q2PP14; Q5CAQ6; Q5CAQ7; Q9BVQ5; A8K500; B3KPJ9
• Molecular Weight: 98,161 Da
• NCBI Official Full Name: Heat shock protein HSP 90-alpha
• NCBI Official Synonym Full Names: heat shock protein 90 alpha family class A member 1
• NCBI Official Symbol: HSP90AA1
• NCBI Official Synonym Symbols: EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; LAP-2; HSP89A; HSP90A; HSP90N; Hsp103; HSPCAL1; HSPCAL4; HEL-S-65p
• NCBI Protein Information: heat shock protein HSP 90-alpha
• UniProt Protein Name: Heat shock protein HSP 90-alpha
• Protein Family: Heat shock protein
• UniProt Gene Name: HSP90AA1
• UniProt Synonym Gene Names: HSP90A; HSPC1; HSPCA; HSP 86; HSP86; LAP-2; LPS-associated protein 2

NCBI Description

The protein encoded by this gene is an inducible molecular chaperone that functions as a homodimer. The encoded protein aids in the proper folding of specific target proteins by use of an ATPase activity that is modulated by co-chaperones. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity which is essential for its chaperone activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function (PubMed:11274138, PubMed:15577939, PubMed:15937123, PubMed:27353360, PubMed:29127155). Engages with a range of client protein classes via its interaction with various co-chaperone proteins or complexes, that act as adapters, simultaneously able to interact with the specific client and the central chaperone itself (PubMed:29127155). Recruitment of ATP and co-chaperone followed by client protein forms a functional chaperone. After the completion of the chaperoning process, properly folded client protein and co-chaperone leave HSP90 in an ADP-bound partially open conformation and finally, ADP is released from HSP90 which acquires an open conformation for the next cycle (PubMed:27295069, PubMed:26991466). Apart from its chaperone activity, it also plays a role in the regulation of the transcription machinery. HSP90 and its co-chaperones modulate transcription at least at three different levels (PubMed:25973397). In the first place, they alter the steady-state levels of certain transcription factors in response to various physiological cues(PubMed:25973397). Second, they modulate the activity of certain epigenetic modifiers, such as histone deacetylases or DNA methyl transferases, and thereby respond to the change in the environment (PubMed:25973397). Third, they participate in the eviction of histones from the promoter region of certain genes and thereby turn on gene expression (PubMed:25973397). Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes (PubMed:11276205). Antagonizes STUB1-mediated inhibition of TGF-beta signaling via inhibition of STUB1-mediated SMAD3 ubiquitination and degradation (PubMed:24613385).