Mouse HSP90 Monoclonal Antibody | anti-HSP90 antibody

HSP90 monoclonal antibody (2D11B9)

Cat. Num. AAA565740

• Gene Names: HSP90AA1; EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; LAP-2; HSP89A; HSP90A; HSP90N; Hsp103; HSPCAL1; HSPCAL4; HEL-S-65p
• Reactivity: Human, Mouse, Rat, Sheep, Dog, Pig, Monkey, Drosophila, Fish, Guinea pig, Hamster, horse
• Applications: Western Blot
• Purity: Protein G affinity purified.
• Synonyms: HSP90; Monoclonal Antibody; HSP90 monoclonal antibody (2D11B9); Heat shock protein 90; mAb (2D11B9); anti-HSP90 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Sheep, Dog, Pig, Monkey, Drosophila, Fish, Guinea pig, Hamster, horse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 2D11B9
• Specificity: Recognizes human, mouse, rat, dog, Drosophila, fish, guinea pig, hamster, horse, monkey, pig, and sheep HSP90. Detects a band of ~92kDa by Western blot.
• Purity/Purification: Protein G affinity purified.
• Form/Format: Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
• Sequence Length: 732

• Applicable Applications for anti-HSP90 antibody: Western Blot (WB)
• Application Notes: Western Blot (1ug/ml, colorimetric).; Detects a band of ~92kDa by Western blot.
• Immunogen: Recombinant human Hsp90.
• Preparation and Storage: Long Term: -20 degree C; Avoid freeze/thaw cycles.

Western Blot (WB)

(Western Blot Analysis of HSP90, mAb (2D11B9). Lane 1: MWM; Lane 2: HSP90 (human), (native); Lane 3: HeLa, (cell lysate); and Lane 4: HeLa (heat shocked), (cell lysate).)

Related Product Information for anti-HSP90 antibody

The 90kDa molecular chaperone family includes 90kDa heat shock protein Hsp90 and 94kDa glucose-regulated protein grp94, both major molecular chaperones of the cytosol and the endoplasmic reticulum. Mammalian cells contain isoforms Hsp90alpha and Hsp90beta, encoded by separate genes. The amino acid sequences of human and yeast Hsp90alpha are 85% and 90% homologous to those of Hsp90beta, respectively. All known members of the Hsp90 protein family are highly conserved, especially in the N-terminal and C-terminal regions containing independent chaperone sites with different substrate specificity. These ubiquitous and highly conserved proteins account for 1-2% of all cellular proteins in most cells. Hsp90 functions as part of the cell’s powerful network of chaperones to fight the deleterious consequences of protein unfolding caused by non-physiological conditions. In the absence of stress, however, Hsp90 provides a necessary component of such fundamental cellular processes as hormone signalling and cell cycle control. In this context, researchers identified key regulatory proteins as substrates of Hsp90, including steroid receptors, cell cycle kinases involved in signal transduction, and p53. Hsp90 may act as a capacitor for morphological evolution by buffering widespread variation, potentially affecting morphogenic pathways. When temperature and other stress factors compromise Drosophilia Hsp90 buffering, cryptic variant expression occurs, and selection can lead to the continued expression of these traits, even after Hsp90 function is restored.

Product Categories/Family for anti-HSP90 antibody

Protein Synthesis & Folding; Proteolysis & Protein Degradation; Cell Death

NCBI and Uniprot Product Information

• NCBI GI #: 92090606
• NCBI GeneID: 3320
• UniProt Accession #: P07900; Q2PP14; Q5CAQ6; Q5CAQ7; Q9BVQ5; A8K500; B3KPJ9
• Molecular Weight: 98,161 Da
• NCBI Official Full Name: Heat shock protein HSP 90-alpha
• NCBI Official Synonym Full Names: heat shock protein 90 alpha family class A member 1
• NCBI Official Symbol: HSP90AA1
• NCBI Official Synonym Symbols: EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; LAP-2; HSP89A; HSP90A; HSP90N; Hsp103; HSPCAL1; HSPCAL4; HEL-S-65p
• NCBI Protein Information: heat shock protein HSP 90-alpha
• UniProt Protein Name: Heat shock protein HSP 90-alpha
• Protein Family: Heat shock protein
• UniProt Gene Name: HSP90AA1
• UniProt Synonym Gene Names: HSP90A; HSPC1; HSPCA; HSP 86; HSP86; LAP-2; LPS-associated protein 2

NCBI Description

The protein encoded by this gene is an inducible molecular chaperone that functions as a homodimer. The encoded protein aids in the proper folding of specific target proteins by use of an ATPase activity that is modulated by co-chaperones. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity which is essential for its chaperone activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes (PubMed:11274138, PubMed:11276205, PubMed:15577939, PubMed:15937123, PubMed:27353360).