Mouse hnRNP A2B1 Monoclonal Antibody | anti-hnRNP A2B1 antibody

Mouse Anti-hnRNP A2B1 [DP3B3]

Cat. Num. AAA375152

• Reactivity: Chicken, Human, Mouse, Rat, Pig
• Applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry, ELISA, Western Blot, Immunoprecipitation
• Purity: Protein A
• Synonyms: hnRNP A2B1; Monoclonal Antibody; Mouse Anti-hnRNP A2B1 [DP3B3]; Heterogeneous nuclear ribonucleoprotein A2 antibody; Heterogeneous nuclear ribonucleoprotein A2/B1antibody; Heterogeneous nuclear ribonucleoprotein B1 antibody; Heterogeneous nuclear ribonucleoproteins A2/B1antibody; hnRNP A2 antibody; hnRNP A2 / hnRNP B1 antibody; hnRNP B1 antibody; hnRNP-A2 antibody; hnRNP-B1antibody; hnRNPA2 antibody; hnRNPB1 antibody; HNRPA2 antibody; HNRPA2B1 antibody; HNRPB1 antibody; Nuclearribonucleoprotein particle A2 protein antibody; RNP A2 antibody; RNP B1 antibody; RNP-A2 antibody; RNP-B1antibody; RNPA2 antibody; RNPB1 antibody; SNRPB1 antibody; anti-hnRNP A2B1 antibody

• Host: Mouse
• Reactivity: Chicken, Human, Mouse, Rat, Pig
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: DP3B3
• Specificity: Reacts predominantly with a 36 kDa band (A2), a 38kDa band (B1)
• Purity/Purification: Protein A
• Form/Format: Purified antibody (from supernatant) containing PBS 0.1% sodium azide
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-hnRNP A2B1 antibody: Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Optimal antibody dilution should be determined by titration, however as a guideline try; IHC-Fr: 1/100. PubMed: 17067748Fix with acetone WB: 1/1500 - 1/6000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa) IHC-P: Use a concentration of 1 ug/ml
• Target Antigen: hnRNP A2B1
• Immunogen: Bacterially expressed His-hnRNP-A2 fusion protein (Human)
• Myeloma/fusion Partners: SP2/0
• Research Areas: RNA binding; RNA Processing

Immunohistochemistry (IHC)

References

Rambaruth ND et al. The lectin Helix pomatia agglutinin recognizes O-GlcNAc containing glycoproteins in human breast cancer. Glycobiology 22:839-48 (2012). WB, ICC/IF ; Human PUBMED Wong PF et al. Eurycomanone suppresses expression of lung cancer cell tumor markers, prohibitin, annexin 1 and endoplasmic reticulum protein 28.Phytomedicine. 2012 Jan 15;19(2):138-44. doi: 10.1016/j.phymed.2011.07.001. Epub 2011 Sep 7.WB ; Human PMID: 21903368 Maeto CA et al. Differential effect of acute and persistent Junin virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B. J Gen Virol 92:2181-90 (2011) PUBMED Clower CV et al. The alternative splicing repressors hnRNP A1/A2 and PTB influence pyruvate kinase isoform expression and cell metabolism. Proc Natl Acad Sci U S A 107:1894-9 (2010). WB ; Human PUBMED Rosenberger S et al. Alternative splicing of human papillomavirus type-16 E6/E6* early mRNA is coupled to EGF signaling via Erk1/2 activation. Proc Natl Acad Sci U S A : (2010). WB ; Human PUBMED Santarosa M et al. BRCA1 modulates the expression of hnRNPA2B1 and KHSRP. Cell Cycle 9:4666-4673 (2010). WB ; Human PUBMED Nicholls CD et al. Multiple factors influence the normal and UV-inducible alternative splicing of PIG3.Biochim Biophys Acta. 2008 Dec;1779(12):838-49. doi: 10.1016/j.bbagrm.2008.08.009. Epub 2008 Aug 29.WB ; Human PMID: 18801469 Lee JH et al. Alterations in Gemin5 expression contribute to alternative mRNA splicing patterns and tumor cell motility. Cancer Res 68:639-44 (2008). WB ; Human PUBMED Honor© B et al. Identification of differentially expressed proteins in spontaneous thymic lymphomas from knockout mice with deletion of p53.Proteome Sci. 2008 Jun 10;6:18. doi: 10.1186/1477-5956-6-18.WB ; Mouse PMID: 18544163 Katherine A. Peebles et al. Altered expression of splicing factor, heterogeneous nuclear ribonucleoprotein A2/B1, in mouse lung neoplasia. Molecular Carcinogenesis,3 May 2007IHC WB ; Mouse PMID: 17477362 Inoue A et al. Loss-of-function screening by randomized intracellular antibodies: identification of hnRNP-K as a potential target for metastasis.Proc Natl Acad Sci U S A. 2007 May 22;104(21):8983-8. Epub 2007 May 4.WB ; Human PMID: 17483488 Griffith BN et al. Identification of hnRNPs K, L and A2/B1 as candidate proteins involved in the nutritional regulation of mRNA splicing.Biochim Biophys Acta. 2006 Nov-Dec;1759(11-12):552-61. Epub 2006 Oct 6.WB ; Mouse PMID: 17095106 Szeszel-Fedorowicz W et al. An exonic splicing silencer is involved in the regulated splicing of glucose 6-phosphate dehydrogenase mRNA.J Biol Chem. 2006 Nov 10;281(45):34146-58. Epub 2006 Sep 15.WB ; Human Rat PMID: 16980303 Strom A et al. Identification of prion protein binding proteins by combined use of far-Western immunoblotting, two dimensional gel electrophoresis and mass spectrometry. Proteomics 6:26-34 (2006) PUBMED Zech VF et al. Prognostic and diagnostic relevance of hnRNP A2/B1, hnRNP B1 and S100 A2 in non-small cell lung cancer. Cancer Detect Prev 30:395-402 (2006). IHC-Fr ; Human PUBMED Iwahashi CK et al. Protein composition of the intranuclear inclusions of FXTAS.Brain. 2006 Jan;129(Pt 1):256-71. Epub 2005 Oct 24.WB ICC/IF ; Human PMID: 16246864 Xia H Regulation of gamma-fibrinogen chain expression by heterogeneous nuclear ribonucleoprotein A1. J Biol Chem 280:13171-8 (2005) PUBMED Hong OK et al. Proteomic analysis of differential protein expression in response to epidermal growth factor in neonatal porcine pancreatic cell monolayers. J Cell Biochem 95:769-81 (2005) PUBMED Molecular characterization of the hnRNP A2/B1 proteins: tissuespecific expression and novel isoforms. Kamma H (Exp Cell Res.,Feb 1999)PUBMED