Mouse hnRNP Q Monoclonal Antibody | anti-hnRNP Q antibody

Mouse Anti-hnRNP Q [I8E4]

Cat. Num. AAA375176

• Gene Names: SYNCRIP; PP68; NSAP1; GRYRBP; HNRNPQ; HNRPQ1; GRY-RBP; hnRNP-Q
• Reactivity: Mouse, Human, Xenopus laevis
• Applications: Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA, Western Blot, Immunoprecipitation
• Purity: Protein A
• Synonyms: hnRNP Q; Monoclonal Antibody; Mouse Anti-hnRNP Q [I8E4]; dJ3J17.2 antibody; Glycine and tyrosine rich RNA binding protein antibody; GRY RBPantibody; Heterogeneous nuclear ribonucleoprotein Q antibody; HNRPQ 1 antibody; HNRPQ antibody; HNRPQ1antibody; NS1 associated protein 1 antibody; NSAP 1 antibody; NSAP1 antibody; pp68 antibody; RP1 3J17.2antibody; Synaptotagmin binding cytoplasmic RNA interacting protein antibody; SYNCRIPantibody; anti-hnRNP Q antibody

• Host: Mouse
• Reactivity: Mouse, Human, Xenopus laevis
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: I8E4
• Purity/Purification: Protein A
• Form/Format: Purified antibody (from supernatant) containing PBS 0.1% sodium azide
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 562

• Applicable Applications for anti-hnRNP Q antibody: Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Optimal antibody dilution should be determined by titration, however as a guideline try; Flow Cyt: Use 1ug for 106 cells. WB: Use at an assay dependent dilution. Detects a band of approximately 55,60,70 kDa (predicted molecular weight: 70 kDa).
• Target Antigen: hnRNP Q
• Immunogen: Recombinant untagged hnRNP-Q protein (human)
• Myeloma/fusion Partners: SP2/0
• Research Areas: RNA binding; RNA Processing; Splicing

Western Blot (WB)

References

Katoh H et al. Japanese Encephalitis Virus Core Protein Inhibits Stress Granule Formation through an Interaction with Caprin-1 and Facilitates Viral Propagation. J Virol 87:489-502 (2013). WBPUBMED Peddigari S et al. hnRNPL and nucleolin bind LINE-1 RNA and function as host factors to modulate retrotransposition. Nucleic Acids Res 41:575-85 (2013). WB ; HumanPUBMED Evans JD et al. Paired mutations abolish and restore the balanced annealing and melting activities of ORF1p that are required for LINE-1 retrotransposition. Nucleic Acids Res 39:5611-21 (2011). WB ; HumanPUBMED Krger M et al. Dissection of the insulin signaling pathway via quantitative phosphoproteomics. Proc Natl Acad Sci U S A 105:2451-6 (2008). WB ; MousePUBMED Fu D & Collins K Purification of human telomerase complexes identifies factors involved in telomerase biogenesis and telomere length regulation. Mol Cell 28:773-85 (2007). IP ; HumanPUBMED Hogg JR & Collins K RNA-based affinity purification reveals 7SK RNPs with distinct composition and regulation. RNA 13:868-80 (2007). WB ; HumanPUBMED Van Herreweghe E et al. Dynamic remodelling of human 7SK snRNP controls the nuclear level of active P-TEFb. EMBO J 26:3570-80 (2007). WB ; HumanPUBMED

NCBI and Uniprot Product Information

• NCBI GI #: 228008400
• NCBI GeneID: 10492
• NCBI Accession #: NP_001153149.1
• NCBI GenBank Nucleotide #: NM_001159677.1
• UniProt Accession #: O60506; Q53H05; Q5TCG2; Q5TCG3; Q8IW78; Q8N599; Q96LC1; Q96LC2; Q9Y583; E1P501; E1P502
• Molecular Weight: 46,328 Da
• NCBI Official Full Name: heterogeneous nuclear ribonucleoprotein Q isoform 6
• NCBI Official Synonym Full Names: synaptotagmin binding, cytoplasmic RNA interacting protein
• NCBI Official Symbol: SYNCRIP
• NCBI Official Synonym Symbols: PP68; NSAP1; GRYRBP; HNRNPQ; HNRPQ1; GRY-RBP; hnRNP-Q
• NCBI Protein Information: heterogeneous nuclear ribonucleoprotein Q; NS1-associated protein 1; glycine- and tyrosine-rich RNA-binding protein
• UniProt Protein Name: Heterogeneous nuclear ribonucleoprotein Q
• UniProt Gene Name: SYNCRIP
• UniProt Synonym Gene Names: HNRPQ; NSAP1; hnRNP Q; GRY-RBP
• UniProt Entry Name: HNRPQ_HUMAN

NCBI Description

This gene encodes a member of the cellular heterogeneous nuclear ribonucleoprotein (hnRNP) family. hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA) and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. The encoded protein plays a role in multiple aspects of mRNA maturation and is associated with several multiprotein complexes including the apoB RNA editing-complex and survival of motor neurons (SMN) complex. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 20. [provided by RefSeq, Dec 2011]

Uniprot Description

NSAP1: a heterogeneous nuclear ribonucleoprotein (hnRNP) implicated in mRNA processing mechanisms. A component of the spliceosome. Five alternatively spliced isoforms have been described. Isoform 1, 2 and 3 are associated with pre-mRNA, splicing intermediates and mature mRNA protein complexes. Isoform 1 binds to apoB mRNA AU-rich sequences. Isoform 1 is part of the APOB mRNA editosome complex and may modulate the postranscriptional C to U RNA-editing of the APOB mRNA through either by binding to ACF (APOBEC1 complementation factor), to APOBEC1 or to RNA itself. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Interacts in vitro preferentially with poly(A) and poly(U) RNA sequences. Isoform 3 may be involved in cytoplasmic vesicle-based mRNA transport through interaction with synaptotagmins.

Protein type: RNA-binding; Motility/polarity/chemotaxis; Spliceosome; RNA splicing

Chromosomal Location of Human Ortholog: 6q14-q15

Cellular Component: nucleoplasm; membrane; endoplasmic reticulum; ribonucleoprotein complex; nucleus

Molecular Function: protein binding; RNA binding; nucleotide binding; poly(A) binding

Biological Process: RNA processing; nuclear mRNA splicing, via spliceosome; osteoblast differentiation; viral reproduction; negative regulation of translation; RNA splicing