Testing Data
(Fig. 1. Typical standard curve for human PARN ELISA (shown for reference only; user must gernerate a new standard curve with each assay).)
Poly A Specific Ribonuclease (PARN) ELISA Kit | PARN elisa kit
Poly A Specific Ribonuclease (PARN) BioAssay ELISA Kit (Human)
Synonyms
Poly A Specific Ribonuclease (PARN); Poly A Specific Ribonuclease (PARN) BioAssay ELISA Kit (Human); PARN elisa kit
Specificity
Human LT-alpha determinates of Human LT-alpha (TNF-beta) in Human serum, Human plasma, cell lysate, culture supernatants and buffered solution.
Samples
Human Serum, Human Plasma, Cell Lysate, Culture Supernatants And Buffered Solution
Assay Type
Quantitative Sandwich
Sensitivity
7.762 pg/ml
Testing Data
(Fig. 1. Typical standard curve for human PARN ELISA (shown for reference only; user must gernerate a new standard curve with each assay).)
Testing Data
(Fig. 1. Typical standard curve for human PARN ELISA (shown for reference only; user must gernerate a new standard curve with each assay).)
Related Product Information for PARN elisa kit
Intended Uses: The Human LT-alpha (TNF-beta) ELISA kit is to be used for the determination of Human LT-alpha (TNF-beta) in Human serum, Human plasma, cell lysate, culture supernatants and buffered solution. The assay will recognize native and recombinant Human LT-alpha (TNF-beta).
Principle of the Assay: The design of this assay is based on a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to Human LT-alpha (TNF-beta). Samples are pipetted into these wells. Nonbound Human LT-alpha (TNF-beta) and other components of the sample should be removed by washing, then monoclonal antibody specific to Human LT-alpha (TNF-beta) added. In order to quantitatively determine the amount of Human LT-alpha (TNF-beta) present in the sample, Streptavidin Horseradish Peroxidase (HRP) should be added to each microplate well. The final step, a TMB-substrate solution added to each well. Finally, a sulfuric acid solution is added and the resulting yellow colored product is measured at 450nm. Since the increases in absorbency is directly proportional to the amount of captured Human LT-alpha (TNF-beta)!!Background/Introduction: Lymphotoxin-alpha (LT-alpha) is a protein that in humans is encoded by the LTA gene. Lymphotoxin alpha, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. LTA is highly inducible, secreted, and exists as homotrimeric molecule. LTA forms heterotrimers with lymphotoxin-beta, which anchors lymphotoxin-alpha to the cell surface. LTA mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. LTA is also involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. In LT-alpha knockout mice, all Peyer's patches and lymph nodes will fail to develop indicating LT-alpha's importance in immunological development. Lymphotoxin alpha has been shown to interact with LTB.
Principle of the Assay: The design of this assay is based on a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to Human LT-alpha (TNF-beta). Samples are pipetted into these wells. Nonbound Human LT-alpha (TNF-beta) and other components of the sample should be removed by washing, then monoclonal antibody specific to Human LT-alpha (TNF-beta) added. In order to quantitatively determine the amount of Human LT-alpha (TNF-beta) present in the sample, Streptavidin Horseradish Peroxidase (HRP) should be added to each microplate well. The final step, a TMB-substrate solution added to each well. Finally, a sulfuric acid solution is added and the resulting yellow colored product is measured at 450nm. Since the increases in absorbency is directly proportional to the amount of captured Human LT-alpha (TNF-beta)!!Background/Introduction: Lymphotoxin-alpha (LT-alpha) is a protein that in humans is encoded by the LTA gene. Lymphotoxin alpha, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. LTA is highly inducible, secreted, and exists as homotrimeric molecule. LTA forms heterotrimers with lymphotoxin-beta, which anchors lymphotoxin-alpha to the cell surface. LTA mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. LTA is also involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. In LT-alpha knockout mice, all Peyer's patches and lymph nodes will fail to develop indicating LT-alpha's importance in immunological development. Lymphotoxin alpha has been shown to interact with LTB.
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Product Notes
The PARN (Catalog #AAA6120662) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Poly A Specific Ribonuclease (PARN), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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