Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Immunofluorescence/Immunocytochemistry (IF/ICC)

Mouse Gemin 1 Monoclonal Antibody

Mouse Anti-Gemin 1 [2B1]

Reactivity
Mouse, Human, Xenopus laevis
Applications
Immunohistochemistry, Immunocytochemistry, Immunofluorescence, ELISA, Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
Protein A
Synonyms
Gemin 1; Monoclonal Antibody; Mouse Anti-Gemin 1 [2B1]; BCD541 antibody; Gemin 1 antibody; Gemin-1 antibody; Gemin1 antibody; OTTHUMP00000125198antibody; OTTHUMP00000223567 antibody; OTTHUMP00000223568 antibody; OTTHUMP00000224066antibody; OTTHUMP00000226924 antibody; SMA 1 antibody; SMA 2 antibody; SMA 3 antibody; SMA 4 antibody; SMAantibody; SMA@ antibody; SMA1 antibody; SMA2 antibody; SMA3 antibody; SMA4 antibody; SMN 1 antibody; SMNantibody; SMN-1 antibody; SMN1 antibody; SMNT antibody; Survival motor neuron protein antibody; Survival ofmotor neuron 1 (telomeric) antibody; survival of motor neuron 1 antibody; Survival of motor neuron 1; telomericantibody; T-BCD541 antibody; anti-Gemin 1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Mouse, Human, Xenopus laevis
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2B1
Purity/Purification
Protein A
Form/Format
Purified antibody (from supernatant) containing PBS + 0.1% sodium azide
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-Gemin 1 antibody
Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
Optimal antibody dilution should be determined by titration, however as a guideline try at IHC-P: Use a concentration of 5 ug/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol WB: Use at an assay dependent dilution. Detects a band of approximately 42 kDa (predicted molecular weight: 35 kDa) Flow Cyt: Use 1ug for 106 cells.
Target Antigen
Gemin 1
Immunogen
Purified recombinant His 6 tagged-SMN protein (human)
Myeloma/fusion Partners
A/J & Sp2/0 cell line

Immunofluorescence/Immunocytochemistry (IF/ICC)

Immunofluorescence/Immunocytochemistry (IF/ICC)
References
Liu, Q. et al. A novel nuclear structure containing the survival of motor neurons protein. The EMBO Journal. Vol.15 no.14: 3555-3565, 1996 PUBMED Grondard C et al. Regular exercise prolongs survival in a type 2 spinal muscular atrophy model mouse. J Neurosci. 2005 Aug 17;25(33):7615-22. IHC ; Human PMID: 16107648

Similar Products

Product Notes

The Gemin 1 (Catalog #AAA375242) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Gemin 1 [2B1] reacts with Mouse, Human, Xenopus laevis and may cross-react with other species as described in the data sheet. AAA Biotech's Gemin 1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). Optimal antibody dilution should be determined by titration, however as a guideline try at IHC-P: Use a concentration of 5 ug/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol WB: Use at an assay dependent dilution. Detects a band of approximately 42 kDa (predicted molecular weight: 35 kDa) Flow Cyt: Use 1ug for 106 cells. Researchers should empirically determine the suitability of the Gemin 1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Gemin 1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.