Mouse HLA A2 Monoclonal Antibody | anti-HLA A2 antibody

MOUSE ANTI HUMAN HLA A2:FITC

Cat. Num. AAA210840

• Gene Names: HLA-A; HLAA
• Applications: Flow Cytometry, Functional Assay
• Synonyms: HLA A2; Monoclonal Antibody; MOUSE ANTI HUMAN HLA A2:FITC; anti-HLA A2 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: BB7.2
• Form/Format: FITC; Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
• Concentration: IgG concentration 0.1 mg/ml (varies by lot)
• Sequence Length: 365

• Applicable Applications for anti-HLA A2 antibody: Flow cytometry (FC/FACS)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood. ; Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10
• Perservative Stabilisers: 0.09% Sodium Azide; 1% Bovine Sodium Albumin; Preparation
• Buffer Solution: Phosphate buffered saline
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human HLA-A2 (MBS212483))

Testing Data

(Staining of human peripheral blood monocytes with Mouse anti Human HLA-A2:Alexa Fluor 488 (MBS212483A488))

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human HLA-A2: endotoxin low (MBS219601))

Testing Data

(Staining of human peripheral blood monocytes with Mouse anti Human HLA-A2:FITC)

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human HLA-A2: RPE (MBS213721))

Related Product Information for anti-HLA A2 antibody

Mouse anti Human HLA A2 antibody, clone BB7.2 recognizes the human HLA-A2 histocompatability antigen. The epitope recognized by this antibody has been studied extensively and would appear to include the carboxy-terminus of the alpha-2 helix and a turn on one of the underlying beta strands. Mouse anti Human HLA A2 antibody, clone BB7.2 may be used for the flow cytometric detection of HLA-A2 expression and has also been used for immunoaffinity purification of HLA-A2 molecules. Functionally Mouse anti Human HLA A2 antibody, clone BB7.2 is reported to inhibit MHC restricted cellular cytotoxicity..

NCBI and Uniprot Product Information

• NCBI GI #: 578841489
• NCBI GeneID: 3105
• NCBI Accession #: XP_006725814.1
• NCBI GenBank Nucleotide #: XM_006725751.1
• UniProt Accession #: P01892; O19619; P06338; P10313; P30444; P30445; P30446; P30514; Q29680; Q29837; Q29899; Q95352; P01891; P04439; P05534; P10314; P10316; P13746; P16189; P18462; P30443
• Molecular Weight: 40,922 Da
• NCBI Official Full Name: HLA class I histocompatibility antigen, A-2 alpha chain isoform X2
• NCBI Official Synonym Full Names: major histocompatibility complex, class I, A
• NCBI Official Symbol: HLA-A
• NCBI Official Synonym Symbols: HLAA
• NCBI Protein Information: HLA class I histocompatibility antigen, A-1 alpha chain; MHC class I antigen HLA-A heavy chain; antigen presenting molecule; leukocyte antigen class I-A
• UniProt Protein Name: HLA class I histocompatibility antigen, A-2 alpha chain
• UniProt Gene Name: HLA-A
• UniProt Synonym Gene Names: HLAA
• UniProt Entry Name: 1A02_HUMAN

NCBI Description

HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

HLAA: Involved in the presentation of foreign antigens to the immune system. Belongs to the MHC class I family.

Protein type: Membrane protein, integral; Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: Golgi membrane; Golgi apparatus; phagocytic vesicle membrane; cell surface; membrane; integral to plasma membrane; early endosome membrane; endoplasmic reticulum; plasma membrane; MHC class I protein complex; Golgi medial cisterna

Molecular Function: protein binding; peptide antigen binding; beta-2-microglobulin binding; T cell receptor binding; TAP binding; receptor binding

Biological Process: regulation of immune response; detection of bacterium; viral reproduction; positive regulation of T cell mediated cytotoxicity; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; regulation of defense response to virus by virus; protection from natural killer cell mediated cytotoxicity; positive regulation of interferon-gamma production; antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent; antigen processing and presentation of peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I; immune response

Disease: Severe Cutaneous Adverse Reaction, Susceptibility To