Testing Data
(Figure A. PE conjugated Mouse anti Human CD3 and FITC conjugated Mouse IgG1 isotype control . Figure B . PE conjugated Mouse anti Human CD3 and FITC conjugated Mouse anti Human HLA-B7 . All experiments performed on human blood gated on single cell lymphocytes in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)
Mouse anti-Human HLA B7 Monoclonal Antibody | anti-HLA-B antibody
Mouse Anti Human HLA B7: Amethyst Orange
Use 10ul of the suggested working dilution to label 1 x 106 cells or 100ul whole blood
1% Bovine Serum Albumin
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody.
This product contains a precipitate we recommend microcentrifugation before use
Testing Data
(Figure A. PE conjugated Mouse anti Human CD3 and FITC conjugated Mouse IgG1 isotype control . Figure B . PE conjugated Mouse anti Human CD3 and FITC conjugated Mouse anti Human HLA-B7 . All experiments performed on human blood gated on single cell lymphocytes in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)
Testing Data
(Figure A. PE conjugated Mouse anti Human CD3 and FITC conjugated Mouse IgG1 isotype control . Figure B . PE conjugated Mouse anti Human CD3 and FITC conjugated Mouse anti Human HLA-B7 . All experiments performed on human blood gated on single cell lymphocytes in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)
Testing Data
(Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Alexa Fluor 647 conjugated Mouse IgG1 isotype control . Figure B . Alexa Fluor 488 conjugated Mouse anti Human CD3 and Alexa Fluor 647 conjugated Mouse anti Human HLA-B7 . All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% Human serum. Data acquired on the ZE5 Cell Analyzer)
Testing Data
(Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Alexa Fluor 647 conjugated Mouse IgG1 isotype control . Figure B . Alexa Fluor 488 conjugated Mouse anti Human CD3 and Alexa Fluor 647 conjugated Mouse anti Human HLA-B7 . All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% Human serum. Data acquired on the ZE5 Cell Analyzer)
Testing Data
(Figure A. Alexa Fluor 700 conjugated Mouse anti Human CD3 and Amethyst Orange conjugated Mouse IgG1 isotype control . Figure B. Alexa Fluor 700 conjugated Mouse anti Human CD3 and Amethyst Orange conjugated Mouse anti Human HLA B7 . All experiments performed on human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)
Testing Data
(Figure A. Alexa Fluor 700 conjugated Mouse anti Human CD3 and Amethyst Orange conjugated Mouse IgG1 isotype control . Figure B. Alexa Fluor 700 conjugated Mouse anti Human CD3 and Amethyst Orange conjugated Mouse anti Human HLA B7 . All experiments performed on human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)
The HLA B gene is part of the human HLA complex on chromosome 6 and there are a large number of variant alleles of this gene.
NCBI and Uniprot Product Information
NCBI Description
HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described. [provided by RefSeq, Jul 2008]
Uniprot Description
HLAB iso7: Involved in the presentation of foreign antigens to the immune system. Belongs to the MHC class I family.
Protein type: Membrane protein, integral; Immunoglobulin superfamily
Chromosomal Location of Human Ortholog: 6p21.3
Cellular Component: Golgi membrane; Golgi apparatus; phagocytic vesicle membrane; cell surface; membrane; early endosome membrane; integral to plasma membrane; endoplasmic reticulum; plasma membrane; integral to membrane; MHC class I protein complex
Molecular Function: peptide antigen binding; chaperone binding; TAP binding; receptor binding
Biological Process: regulation of immune response; regulation of interleukin-12 production; detection of bacterium; viral reproduction; positive regulation of T cell mediated cytotoxicity; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; defense response; protection from natural killer cell mediated cytotoxicity; antigen processing and presentation of peptide antigen via MHC class I; antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent; regulation of T cell anergy; regulation of interleukin-6 production; antigen processing and presentation of exogenous peptide antigen via MHC class I; innate immune response; immune response
Research Articles on HLA-B
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Product Notes
The HLA-B hla-b (Catalog #AAA225864) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti Human HLA B7: Amethyst Orange reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HLA B7 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: Min. Dilution: Neat Use 10ul of the suggested working dilution to label 1 x 106 cells or 100ul whole blood. Researchers should empirically determine the suitability of the HLA-B hla-b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HLA B7, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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