Western Blot (WB)
(Western blot analysis of his-tagged fusion protein.Each lane was loaded with 5ng of his-tagged fusion protein.Lane 1-6: 1: 0.5k, 1: 1k, 1: 2k, 1: 5k, 1: 10k, 1: 20k)
Mouse anti-All His-Tag Monoclonal Antibody
His-Tag mAb (HRP Conjugated)
Reactivity
All
Applications
Western Blot
Synonyms
His-Tag; Monoclonal Antibody; His-Tag mAb (HRP Conjugated); anti-His-Tag antibody
Host
Mouse
Reactivity
All
Clonality
Monoclonal
Isotype
IgG1
Specificity
His-Tag mAb (HRP Conjugated) detects over-expressed or recombinant proteins containingthe His eptiope tag.
Applicable Applications for anti-His-Tag antibody
Western Blot (WB)
Application Notes
WB: 1:5000
Source Note
This monoclonal antibody is produced by immunizing mice with a 6x His synthetic peptide (KLH-coupled).
Preparation and Storage
Store at -20°C from the date of shipment.
Western Blot (WB)
(Western blot analysis of his-tagged fusion protein.Each lane was loaded with 5ng of his-tagged fusion protein.Lane 1-6: 1: 0.5k, 1: 1k, 1: 2k, 1: 5k, 1: 10k, 1: 20k)
Western Blot (WB)
(Western blot analysis of his-tagged fusion protein.Each lane was loaded with 5ng of his-tagged fusion protein.Lane 1-6: 1: 0.5k, 1: 1k, 1: 2k, 1: 5k, 1: 10k, 1: 20k)
Related Product Information for anti-His-Tag antibody
His-Tag mAb (HRP Conjugated) is a monoclonal anti-His antibody covalently linked to horseradish peroxidase.
Product Categories/Family for anti-His-Tag antibody
References
1. Maniattis, T., et al. 1982. Molecular Cloning. Cold Spring Laboratory, Cold Spring Harbor, NY. 2. Hochuli, E. 1988. Large-scale chromatography of recombinant proteins. J.Chromatog. 444: 293-302.
Similar Products
Product Notes
The His-Tag (Catalog #AAA435072) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The His-Tag mAb (HRP Conjugated) reacts with All and may cross-react with other species as described in the data sheet. AAA Biotech's His-Tag can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:5000. Researchers should empirically determine the suitability of the His-Tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "His-Tag, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
