Testing Data
Mouse anti-General His-Tag Monoclonal Antibody
His-Tag Antibody (Clone 11E12)
Reactivity
General
Applications
Western Blot, ELISA
Purity
Protein A chromatography from in vitro production
Synonyms
His-Tag; Monoclonal Antibody; His-Tag Antibody (Clone 11E12); anti-His-Tag antibody
Host
Mouse
Reactivity
General
Clonality
Monoclonal
Isotype
IgG1 kappa
Clone Number
11E12
Purity/Purification
Protein A chromatography from in vitro production
Form/Format
1 mg/ml in 0.15 M PBS
Appearance: Colorless liquid
Appearance: Colorless liquid
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-His-Tag antibody
Western Blot (WB), ELISA (EIA)
Application Notes
ELISA, WB
Immunogen
6X-HIS-KLH
Positive Control
Western blot: His-Tagged protein
Handling
The antibody solution should be gently mixed before use.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Testing Data
Testing Data
Related Product Information for anti-His-Tag antibody
Monoclonal Antibody to detect His-taaged proteins
Background: A variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine (His) residues, followed by an extended multiple cloning sites. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography. As is the case with other protein tags, this polyhistidine tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest.
Background: A variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine (His) residues, followed by an extended multiple cloning sites. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography. As is the case with other protein tags, this polyhistidine tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest.
Product Categories/Family for anti-His-Tag antibody
Similar Products
Product Notes
The His-Tag (Catalog #AAA840047) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The His-Tag Antibody (Clone 11E12) reacts with General and may cross-react with other species as described in the data sheet. AAA Biotech's His-Tag can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). ELISA, WB. Researchers should empirically determine the suitability of the His-Tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "His-Tag, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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