Western Blot (WB)
(Western blot analysis of over-expressed HA-tagged protein in 293T cell using HA antibody at different dilution. Each lane was loaded with 2 ug cell lysate.)
Mouse HA-Tag Monoclonal Antibody | anti-HA-Tag antibody
HA-Tag Monoclonal Antibody
Applications
Western Blot, Immunofluorescence, Immunoprecipitation
Purity
Affinity Purification
Synonyms
HA-Tag; Monoclonal Antibody; HA-Tag Monoclonal Antibody; Mouse anti HA mAb: N/A; anti-HA-Tag antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Concentration
1mg/ml (varies by lot)
Sequence Length
9
Applicable Applications for anti-HA-Tag antibody
Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
WB: 1:2000 - 1:5000
IF: 1:50 - 1:500
IP: 1:50 - 1:100
IF: 1:50 - 1:500
IP: 1:50 - 1:100
Immunogen
A synthetic peptide of YPYDVPDYA (HA-tag)-KLH
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Western Blot (WB)
(Western blot analysis of over-expressed HA-tagged protein in 293T cell using HA antibody at different dilution. Each lane was loaded with 2 ug cell lysate.)
Western Blot (WB)
(Western blot analysis of over-expressed HA-tagged protein in 293T cell using HA antibody at different dilution. Each lane was loaded with 2 ug cell lysate.)
Immunofluorescence (IF)
(Immunofluorescence analysis of over-expressed HA-tagged protein in HeLa cells using HA antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
Immunofluorescence (IF)
(Immunofluorescence analysis of over-expressed HA-tagged protein in HeLa cells using HA antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
Related Product Information for anti-HA-Tag antibody
Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. For example, the pCDM8 expression vector and derivatives thereof encode fusions between the target protein and an 11 amino acid peptide derived from the influenza protein hemagglutinin (HA). The HA epitope tag is useful in Western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-epitope tag.
Product Categories/Family for anti-HA-Tag antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
HA tag, partial
Similar Products
Product Notes
The HA-Tag (Catalog #AAA125004) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HA-Tag can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:2000 - 1:5000 IF: 1:50 - 1:500 IP: 1:50 - 1:100. Researchers should empirically determine the suitability of the HA-Tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HA-Tag, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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