Immunofluorescence (IF)
(Immunofluorescence analysis of HEK293 cells transfected with His-tagged fusion protein, stained with anti-His (red) and DAPI (blue). left: untransfected. Right; transfected.)
Mouse His Tag Monoclonal Antibody | anti-His antibody
His Tag Mouse Monoclonal Antibody (HIS.H8)
Applications
ELISA, Immunoprecipitation, Immunohistochemistry, Western Blot
Synonyms
His Tag; Monoclonal Antibody; His Tag Mouse Monoclonal Antibody (HIS.H8); anti-His antibody
Host
Mouse
Clonality
Monoclonal
Isotype
lgG2b
Clone Number
HIS.H8
Specificity
The antibody recognizes His-tag encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e. reacts with N-terminal, C-terminal or internal 6x His-tags)
Form/Format
Lyophilized protein G purified in PBS pH7.4
Applicable Applications for anti-His antibody
ELISA (EIA), Dot, Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
If reconstituted in 100 muL: WB: 1:1000-3,000; IHC: 1:500-2,000.
Immunogen
The antibody was raised against HHHHHH (6x His) synthetic peptide conjugated to KLH.
Preservative
None
Preparation and Storage
Lyophilized antibody can be kept at 4 degree C for up to 3 months and should be kept at-20 degree C for long-term storage.
To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20 degree C) or for long-term storage (-80 degree C).
For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20 degree C) or for long-term storage (-80 degree C).
For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Immunofluorescence (IF)
(Immunofluorescence analysis of HEK293 cells transfected with His-tagged fusion protein, stained with anti-His (red) and DAPI (blue). left: untransfected. Right; transfected.)
Immunofluorescence (IF)
(Immunofluorescence analysis of HEK293 cells transfected with His-tagged fusion protein, stained with anti-His (red) and DAPI (blue). left: untransfected. Right; transfected.)
Western Blot (WB)
(Western blot analysis of His-tag on standard ladder containing fIVe different HIs-lagged proteins; untransfected control (1), HEK293 cells transfected with His-tagged protein vector (2).)
Western Blot (WB)
(Western blot analysis of His-tag on standard ladder containing fIVe different HIs-lagged proteins; untransfected control (1), HEK293 cells transfected with His-tagged protein vector (2).)
Related Product Information for anti-His antibody
Epitope tags have applications in the labeling, isolation and detection of proteins using immunoblotting, Immunoprecipitation and immunostaining techniques. Epitope tags can be used, by affinity chromatography, to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. Due its small size, the protein's biochemical properties seem not to be affected by the tag protein. Polyhistidine-tag is also known as histidine-tag, 6xHis-tag and by the trademarked name His-tag.
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Product Notes
The His (Catalog #AAA588047) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's His Tag can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Dot, Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blot (WB). If reconstituted in 100 muL: WB: 1:1000-3,000; IHC: 1:500-2,000. Researchers should empirically determine the suitability of the His for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "His Tag, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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