Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat D (2) dopamine receptor (DRD2) Standard Curve.)
Rat D (2) Dopamine Receptor (DRD2) ELISA Kit | DRD2 elisa kit
Rat D (2) Dopamine Receptor (DRD2) ELISA Kit
Reactivity
Rat
Synonyms
D (2) Dopamine Receptor (DRD2); Rat D (2) Dopamine Receptor (DRD2) ELISA Kit; DRD2; D2DR; D2R; dopamine receptor D2 isoform; seven transmembrane helix receptor; DRD2 elisa kit
Reactivity
Rat
Specificity
Rat D (2) dopamine receptor (DRD2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DRD2. No significant cross-reactivity or interference between Rat DRD2 and analogues was observed.
Sequence Length
444
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat D (2) dopamine receptor (DRD2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat D (2) dopamine receptor (DRD2) Standard Curve.)
Related Product Information for DRD2 elisa kit
Description: This Rat D (2) dopamine receptor (DRD2) ELISA Kit employs a two-site sandwich ELISA to quantitate DRD2 in samples. An antibody specific for DRD2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DRD2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DRD2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DRD2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: D2 (DRD2). There is a short version of D2 (D2Sh) and a long version of D2 (D2Lh): The D2Sh are pre-synaptic situated, having modulatory functions (called autoreceptor, they regulate the neurotransmission by feed-back mechanisms, i.e., synthesis, storage and release of dopamine into the synaptic cleft).The D2Lh may have the classic function of a post-synaptic receptor, i.e., keep going on the neurotransmission (excitatory or inhibitory) once blocked by a receptor antagonist or stimulated by the endogenous neurotransmitter itself or a synthetic full or partial agonist.D2-like activation is coupled to the G protein Galphai, which subsequently increases phosphodiesterase activity. Phosphodiesterases break down cAMP, producing an inhibitory effect in neurons.
Background: D2 (DRD2). There is a short version of D2 (D2Sh) and a long version of D2 (D2Lh): The D2Sh are pre-synaptic situated, having modulatory functions (called autoreceptor, they regulate the neurotransmission by feed-back mechanisms, i.e., synthesis, storage and release of dopamine into the synaptic cleft).The D2Lh may have the classic function of a post-synaptic receptor, i.e., keep going on the neurotransmission (excitatory or inhibitory) once blocked by a receptor antagonist or stimulated by the endogenous neurotransmitter itself or a synthetic full or partial agonist.D2-like activation is coupled to the G protein Galphai, which subsequently increases phosphodiesterase activity. Phosphodiesterases break down cAMP, producing an inhibitory effect in neurons.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
D(2) dopamine receptor
NCBI Official Synonym Full Names
dopamine receptor D2
NCBI Official Symbol
Drd2
NCBI Protein Information
D(2) dopamine receptor
UniProt Protein Name
D(2) dopamine receptor
Protein Family
UniProt Gene Name
Drd2
NCBI Description
mediates G-protein coupled signaling; may be involved in movement disorders, schizophrenia, and drug addiction [RGD, Feb 2006]
Uniprot Description
Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase.
Research Articles on DRD2
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Product Notes
The Rat DRD2 drd2 (Catalog #AAA9712585) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712585 ELISA Kit recognizes Rat DRD2. It is sometimes possible for the material contained within the vial of "D (2) Dopamine Receptor (DRD2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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