Western Blot (WB)
(Western blot analysis of HEK29J cells transfected with HA-tagged protein vector. 1:1000 (1mug/ml) Ab dilution Untransfected (1) and transfected (2).)
Mouse HA Tag Monoclonal Antibody | anti-HA antibody
HA Tag Mouse Monoclonal Antibody (HA.C5)
Applications
ELISA, Immunoprecipitation, Immunohistochemistry, Western Blot
Synonyms
HA Tag; Monoclonal Antibody; HA Tag Mouse Monoclonal Antibody (HA.C5); anti-HA antibody
Host
Mouse
Clonality
Monoclonal
Isotype
lgG3
Clone Number
HA.C5
Specificity
The antibody recognizes the HA epitope (YPYDVPDYA) on N-terminal, C-terminal or internal HA-tagged fusion proteins.
Form/Format
Lyophilized protein G purified in PBS pH7.4
Sequence Length
9
Applicable Applications for anti-HA antibody
ELISA (EIA), Dot, Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
If reconstituted in 100 muL: WB: 1:1000-3,000; IHC: 1:500-2,000.
Immunogen
The antibody was raised against YPYDVPDYA (HA) synthetic peptide conjugated to KLH.
Preservative
None
Preparation and Storage
Lyophilized antibody can be kept at 4 degree C for up to 3 months and should be kept at-20 degree C for long-term storage.
To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20 degree C) or for long-term storage (-80 degree C).
For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20 degree C) or for long-term storage (-80 degree C).
For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of HEK29J cells transfected with HA-tagged protein vector. 1:1000 (1mug/ml) Ab dilution Untransfected (1) and transfected (2).)
Western Blot (WB)
(Western blot analysis of HEK29J cells transfected with HA-tagged protein vector. 1:1000 (1mug/ml) Ab dilution Untransfected (1) and transfected (2).)
Related Product Information for anti-HA antibody
Epitope tags have application in the labeling, isolation and detection of proteins using immunoblotting, Immunoprecipitation and immunostaining techniques. Epitope tags can be used, by affinity chromatography, to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. Due its small size the protein's biochemical properties seem not to be affected by the tag protein. Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of human by the virus. The HA tag corresponds to the amino acids 98-106 (YPYDVPDYA) of the HA protein. It has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag.
NCBI and Uniprot Product Information
Similar Products
Product Notes
The HA (Catalog #AAA588208) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HA Tag can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Dot, Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blot (WB). If reconstituted in 100 muL: WB: 1:1000-3,000; IHC: 1:500-2,000. Researchers should empirically determine the suitability of the HA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HA Tag, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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