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Western Blot (WB)
(Western Blot analysis of 0.5ug GST fusion protein using GST-Tag Monoclonal Antibody at dilution of 1) 1:5000 2) 1:10000.)
Epitope Tag Antibody Kit
Epitope Tag Antibody Sampler Kit
Applications
Western Blot
Synonyms
Epitope Tag; Epitope Tag Antibody Sampler Kit; Epitope Tag antibody kit
Applicable Applications for Epitope Tag antibody kit
Western Blot (WB)
Western Blot (WB)
(Western Blot analysis of 0.5ug GST fusion protein using GST-Tag Monoclonal Antibody at dilution of 1) 1:5000 2) 1:10000.)
Western Blot (WB)
(Western Blot analysis of 0.5ug GST fusion protein using GST-Tag Monoclonal Antibody at dilution of 1) 1:5000 2) 1:10000.)
Western Blot (WB)
(Western Blot analysis of 1ug Myc fusion protein using Myc-Tag Monoclonal Antibody at dilution of 1) 1:5000 2) 1:10000.)
Western Blot (WB)
(Western Blot analysis of 1ug Myc fusion protein using Myc-Tag Monoclonal Antibody at dilution of 1) 1:5000 2) 1:10000.)
Western Blot (WB)
(Western Blot analysis of 0.5ug HA fusion protein using HA-Tag Monoclonal Antibody at dilution of 1:10000.)
Western Blot (WB)
(Western Blot analysis of 0.5ug HA fusion protein using HA-Tag Monoclonal Antibody at dilution of 1:10000.)
Western Blot (WB)
(Western Blot analysis of 2ug His fusion protein using His-Tag Monoclonal Antibody at dilution of 1) 1:5000 2) 1:10000.)
Western Blot (WB)
(Western Blot analysis of 2ug His fusion protein using His-Tag Monoclonal Antibody at dilution of 1) 1:5000 2) 1:10000.)
Western Blot (WB)
(Western Blot analysis of 1ug DYKDDDDK fusion protein using DYKDDDDK-tag Monoclonal Antibody at dilution of 1:10000.)
Western Blot (WB)
(Western Blot analysis of 1ug DYKDDDDK fusion protein using DYKDDDDK-tag Monoclonal Antibody at dilution of 1:10000.)
Related Product Information for Epitope Tag antibody kit
Background: Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. Several different epitope tags are now commonly utilized and readily available. For instance, a variety of plasmids contain DNA that encodes an amino-terminal tag consisting of six histidine (6xHis) residues followed by an extended multiple cloning site. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni2+ affinity resins and purification by single step chromatography. As is the case with other protein tag systems, this polyhistidine tag can often be cleaved at sites recognized by proteases such as thrombin and enterokinases to isolate the protein of interest. Glutathione S-transferase (GST) is another widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade. The HA tag, derived from an epitope of the influenza hemagglutinin protein, has also been extensively used as a general epitope tag in expression vectors, while the Myc epitope tag is routinely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems. Finally, the DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors and consists of only eight amino acids. This peptide can be expressed and detected with the protein of interested as an amino- terminal or carboxy-terminal fusion.
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Product Notes
The Epitope Tag (Catalog #AAA2550081) is an Antibody Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Epitope Tag can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the Epitope Tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Epitope Tag, Antibody Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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