Western Blot
(L02 cells were subjected to SDS PAGE followed by western blot with AMIGO3 antibody at dilution of 1:300 predicted band size : 55kDa observed band size : 55kDa)
Rabbit adhesion molecule with Ig-like domain 3 Polyclonal Antibody | anti-AMIGO3 antibody
Rabbit anti-human adhesion molecule with Ig-like domain 3 polyclonal Antibody
Gene Names
AMIGO3; ALI3; AMIGO-3
Reactivity
Human,Mouse,Rat; Other species are not tested. Please decide the specificity by homology.
Applications
ELISA, Western Blot
Purity
Antigen Affinity Purified
Synonyms
adhesion molecule with Ig-like domain 3; Polyclonal Antibody; Rabbit anti-human adhesion molecule with Ig-like domain 3 polyclonal Antibody; AMIGO3; MGC120552; anti-AMIGO3 antibody
Host
Rabbit
Reactivity
Human,Mouse,Rat; Other species are not tested. Please decide the specificity by homology.
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid
Sequence Length
504
Applicable Applications for anti-AMIGO3 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
ELISA: Use at an assay dependent dilution.
WB : 1:500-1:5000(Recommender dilutions).
Positive WB detected in : MCF7 cells, HeLa cells, human brain tissue, mouse kidney tissue, mouse ovary tissue, L02 cells.
WB : 1:500-1:5000(Recommender dilutions).
Positive WB detected in : MCF7 cells, HeLa cells, human brain tissue, mouse kidney tissue, mouse ovary tissue, L02 cells.
Immunogen
Human AMIGO3
Conjugate
Non-conjugated
Storage Buffer
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-49881 / sc-49882 / sc-292657
Preparation and Storage
Shipped at 4 Degree C. Upon delivery aliquot and store at -20 Degree C or -80 Degree C. Avoid repeated freeze.
Western Blot
(L02 cells were subjected to SDS PAGE followed by western blot with AMIGO3 antibody at dilution of 1:300 predicted band size : 55kDa observed band size : 55kDa)
Western Blot
(L02 cells were subjected to SDS PAGE followed by western blot with AMIGO3 antibody at dilution of 1:300 predicted band size : 55kDa observed band size : 55kDa)
Western Blot2
(MCF7 cells were subjected to SDS PAGE followed by western blot with AMIGO3 antibody at dilution of 1:300 predicted band size :55kDa observed band size :55kDa )
Western Blot2
(MCF7 cells were subjected to SDS PAGE followed by western blot with AMIGO3 antibody at dilution of 1:300 predicted band size :55kDa observed band size :55kDa )
Western Blot3
(mouse kidney tissue were subjected to SDS PAGE followed by western blot with AMIGO3 antibody at dilution of 1:300 predicted band size :55kDa observed band size :55kDa )
Western Blot3
(mouse kidney tissue were subjected to SDS PAGE followed by western blot with AMIGO3 antibody at dilution of 1:300 predicted band size :55kDa observed band size :55kDa )
Related Product Information for anti-AMIGO3 antibody
May mediate heterophilic cell-cell interaction. May contribute to signal transduction through its intracellular domain
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
55,250 Da
NCBI Official Full Name
Adhesion molecule with Ig-like domain 3
NCBI Official Synonym Full Names
adhesion molecule with Ig-like domain 3
NCBI Official Symbol
AMIGO3
NCBI Official Synonym Symbols
ALI3; AMIGO-3
NCBI Protein Information
amphoterin-induced protein 3; AMIGO3; alivin-3; amphoterin-induced gene and ORF 3
UniProt Protein Name
Amphoterin-induced protein 3
Protein Family
UniProt Gene Name
AMIGO3
UniProt Entry Name
AMGO3_HUMAN
Uniprot Description
AMIGO3: May mediate heterophilic cell-cell interaction. May contribute to signal transduction through its intracellular domain. Belongs to the immunoglobulin superfamily. AMIGO family.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 3p21
Cellular Component: integral to membrane
Biological Process: heterophilic cell adhesion; brain development
Research Articles on AMIGO3
Similar Products
Product Notes
The AMIGO3 amigo3 (Catalog #AAA711734) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human adhesion molecule with Ig-like domain 3 polyclonal Antibody reacts with Human,Mouse,Rat; Other species are not tested. Please decide the specificity by homology. and may cross-react with other species as described in the data sheet. AAA Biotech's adhesion molecule with Ig-like domain 3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). ELISA: Use at an assay dependent dilution. WB : 1:500-1:5000(Recommender dilutions). Positive WB detected in : MCF7 cells, HeLa cells, human brain tissue, mouse kidney tissue, mouse ovary tissue, L02 cells. . Researchers should empirically determine the suitability of the AMIGO3 amigo3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "adhesion molecule with Ig-like domain 3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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