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Western Blot (WB) (Western Blot shows lysates of human cartilage tissue. PVDF membrane was probed with MBS608379 (2ug/ml) folllowed by IgG (HRP) anti-mouse secondary antibody. A specific band was detected for Fibromodulin at ~55kD (as indicated). This experiment was conducted under reducing conditions.)

Mouse anti-Human Fibromodulin Monoclonal Antibody | anti-FMOD antibody

Fibromodulin (FMOD, FM, Collagen-binding 59kD Protein)

Gene Names
FMOD; SLRR2E; FMOD
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Synonyms
Fibromodulin; Monoclonal Antibody; Fibromodulin (FMOD; FM; Collagen-binding 59kD Protein); Anti -Fibromodulin (FMOD; anti-FMOD antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2B
Clone Number
9L829
Specificity
Recognizes human Fibromodulin. Species sequence homology: mouse, rat (96%).
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS.
Applicable Applications for anti-FMOD antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 0.5-1ug/ml
Western Blot: 2ug/ml
Immunogen
NS0-derived, recombinant human FMOD (aa76-376; Accession # NP_002014).
Hybridoma
Mouse myeloma cells with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human FMOD (aa76-376).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot shows lysates of human cartilage tissue. PVDF membrane was probed with MBS608379 (2ug/ml) folllowed by IgG (HRP) anti-mouse secondary antibody. A specific band was detected for Fibromodulin at ~55kD (as indicated). This experiment was conducted under reducing conditions.)

Western Blot (WB) (Western Blot shows lysates of human cartilage tissue. PVDF membrane was probed with MBS608379 (2ug/ml) folllowed by IgG (HRP) anti-mouse secondary antibody. A specific band was detected for Fibromodulin at ~55kD (as indicated). This experiment was conducted under reducing conditions.)
Related Product Information for anti-FMOD antibody
Fibromodulin (FMOD) is a Class II small leucine-rich proteoglycan (SLRP) superfamily member. The ~50-70kD protein contains a sulfated N-terminal complement C1q binding domain (aa19-98) and 12 leucine-rich repeats (aa75-376). A 40kD form is reported in dental tissues. It participates in collagen fibrillogenesis in many tissues and competes with Lumican for collagen binding. It can participate in inflammation by activating complement. In epidermal keratinocytes, it may be located in the cytoplasm. The region of human FMOD used as an immunogen shares 96% aa identity with mouse or rat FMOD.
Product Categories/Family for anti-FMOD antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
43,179 Da
NCBI Official Full Name
fibromodulin
NCBI Official Synonym Full Names
fibromodulin
NCBI Official Symbol
FMOD
NCBI Official Synonym Symbols
SLRR2E; FMOD
NCBI Protein Information
fibromodulin; FM; KSPG fibromodulin; fibromodulin proteoglycan; collagen-binding 59 kDa protein; keratan sulfate proteoglycan fibromodulin
UniProt Protein Name
Fibromodulin
Protein Family
UniProt Gene Name
FMOD
UniProt Synonym Gene Names
FM; SLRR2E
UniProt Entry Name
FMOD_HUMAN

NCBI Description

Fibromodulin is a member of a family of small interstitial proteoglycans, containing a central region composed of leucine-rich repeats with 4 keratan sulfate chains flanked by disulfide-bonded terminal domains. It may participate in the assembly of the extracellular matrix as it interacts with type I and type II collagen fibrils and inhibits fibrillogenesis in vitro. It may also regulate TGF-beta activities by sequestering TGF-beta into the extracellular matrix. [provided by RefSeq]

Uniprot Description

FMOD: Affects the rate of fibrils formation. May have a primary role in collagen fibrillogenesis. Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: extracellular matrix; lysosomal lumen; proteinaceous extracellular matrix; extracellular space; Golgi lumen; extracellular region

Biological Process: transforming growth factor beta receptor complex assembly; keratan sulfate metabolic process; extracellular matrix organization and biogenesis; glycosaminoglycan metabolic process; carbohydrate metabolic process; keratan sulfate biosynthetic process; pathogenesis; keratan sulfate catabolic process

Research Articles on FMOD

Similar Products

Product Notes

The FMOD fmod (Catalog #AAA608379) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Fibromodulin (FMOD, FM, Collagen-binding 59kD Protein) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Fibromodulin can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 0.5-1ug/ml Western Blot: 2ug/ml. Researchers should empirically determine the suitability of the FMOD fmod for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Fibromodulin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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