Western Blot (WB)
(Western Blot: Sample: Lane1: Human HeLa Cells; Lane2: Human HepG2 Cells; Lane3: Porcine Brain Tissue; Lane4: Mouse Brain Tissue.)
Rabbit anti-Human Phosphoserine Aminotransferase 1 Polyclonal Antibody | anti-PSAT1 antibody
Polyclonal Antibody to Phosphoserine Aminotransferase 1 (PSAT1)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MDAPRQVVNF GPGPAKLPHS VLLEIQKELL DYKGVGISVL EMSHRSSDFA KIINNTENLV RELLAVPDNY KVIFLQGGGC GQFSAVPLNL IGLKAGRCAD YVVTGAWSAK AAEEAKKFGT INIVHPKLGS YTKIPDPSTW NLNPDASYVY YCANETVHGV EFDFIPDVKG AVLVCDMSSN FLSKPVDVSK FGVIFAGAQK NVGSAGVTVV IVRDDLLGFA LRECPSVLEY KVQAGNSSLY NTPPCFSIYV MGLVLEWIKN
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Sample: Lane1: Human HeLa Cells; Lane2: Human HepG2 Cells; Lane3: Porcine Brain Tissue; Lane4: Mouse Brain Tissue.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Human HeLa Cells; Lane2: Human HepG2 Cells; Lane3: Porcine Brain Tissue; Lane4: Mouse Brain Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant PSAT1, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant PSAT1, Human.)
Immunohistochemistry (IHC)
(FITC staining on IHC-P; Samples: Human Hela Cells))
Immunohistochemistry (IHC)
(FITC staining on IHC-P; Samples: Human Hela Cells))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Brain Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Brain Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue)
Knockout Validation
(Knockout Validation: Lane 1: Wild-type Hela cell lysate; Lane 2: PSAT1 knockout Hela cell lysate; Predicted MW: 40kd Observed MW: 40kd Primary Ab: 2ug/ml Rabbit Anti-Human PSAT1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type Hela cell lysate; Lane 2: PSAT1 knockout Hela cell lysate; Predicted MW: 40kd Observed MW: 40kd Primary Ab: 2ug/ml Rabbit Anti-Human PSAT1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the class-V pyridoxal-phosphate-dependent aminotransferase family. The encoded protein is a phosphoserine aminotransferase and decreased expression may be associated with schizophrenia. Mutations in this gene are also associated with phosphoserine aminotransferase deficiency. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene have been defined on chromosomes 1, 3, and 8. [provided by RefSeq, Jul 2013]
Uniprot Description
PSAT1: Catalyzes the reversible conversion of 3- phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4- phosphonooxybutanoate to phosphohydroxythreonine. Defects in PSAT1 are the cause of phosphoserine aminotransferase deficiency (PSATD). PSATD is characterized biochemically by low plasma and cerebrospinal fluid concentrations of serine and glycine and clinically by intractable seizures, acquired microcephaly, hypertonia, and psychomotor retardation. Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Amino Acid Metabolism - glycine, serine and threonine; Cofactor and Vitamin Metabolism - vitamin B6; EC 2.6.1.52; Transferase
Chromosomal Location of Human Ortholog: 9q21.2
Cellular Component: cytoplasm; cytosol; extracellular exosome
Molecular Function: phosphoserine transaminase activity
Biological Process: L-serine biosynthetic process; pyridoxine biosynthetic process
Disease: Neu-laxova Syndrome 2; Phosphoserine Aminotransferase Deficiency
Research Articles on PSAT1
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Product Notes
The PSAT1 psat1 (Catalog #AAA2028385) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Phosphoserine Aminotransferase 1 (PSAT1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Phosphoserine Aminotransferase 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-2ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the PSAT1 psat1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MDAPRQVVNF GPGPAKLPHS VLLEIQKELL DYKGVGISVL EMSHRSSDFA KIINNTENLV RELLAVPDNY KVIFLQGGGC GQFSAVPLNL IGLKAGRCAD YVVTGAWSAK AAEEAKKFGT INIVHPKLGS YTKIPDPSTW NLNPDASYVY YCANETVHGV EFDFIPDVKG AVLVCDMSSN FLSKPVDVSK FGVIFAGAQK NVGSAGVTVV IVRDDLLGFA LRECPSVLEY KVQAGNSSLY NTPPCFSIYV MGLVLEWIKN. It is sometimes possible for the material contained within the vial of "Phosphoserine Aminotransferase 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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