Western Blot (WB)
(GCA monoclonal antibody (M04), clone 2F11. Western Blot analysis of GCA expression in IMR-32.)
Mouse GCA Monoclonal Antibody | anti-GCA antibody
GCA (Grancalcin, EF-hand Calcium Binding Protein, GCL) (APC)
Gene Names
GCA; GCL
Applications
Western Blot
Purity
Purified
Synonyms
GCA; Monoclonal Antibody; GCA (Grancalcin; EF-hand Calcium Binding Protein; GCL) (APC); Grancalcin; GCL; anti-GCA antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
2F11
Specificity
Recognizes GCA.
Purity/Purification
Purified
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Sequence Length
217
Applicable Applications for anti-GCA antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
GCA (AAH05214, 1aa-217aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MAYPGYGGGFGNFSIQVPGMQMGQPVPETGPAILLDGYSGPAYSDTYSSAGDSVYTYFSAVAGQDGEVDAEELQRCLTQSGINGTYSPFSLETCRIMIAMLDRDHTGKMGFNAFKELWAALNAWKENFMTVDQDGSGTVEHHELRQAIGLMGYRLSPQTLTTIVKRYSKNGRIFFDDYVACCVKLRALTDFFRKRDHLQQGSANFIYDDFLQGTMAI
Conjugate
APC
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(GCA monoclonal antibody (M04), clone 2F11. Western Blot analysis of GCA expression in IMR-32.)
Western Blot (WB)
(GCA monoclonal antibody (M04), clone 2F11. Western Blot analysis of GCA expression in IMR-32.)
Testing Data
(Detection limit for recombinant GST tagged GCA is 0.03 ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged GCA is 0.03 ng/ml as a capture antibody.)
Related Product Information for anti-GCA antibody
This gene product, grancalcin, is a calcium-binding protein abundant in neutrophils and macrophages. It belongs to the penta-EF-hand subfamily of proteins which includes sorcin, calpain, and ALG-2. Grancalcin localization is dependent upon calcium and magnesium. In the absence of divalent cation, grancalcin localizes to the cytosolic fraction; with magnesium alone, it partitions with the granule fraction; and in the presence of magnesium and calcium, it associates with both the granule and membrane fractions, suggesting a role for grancalcin in granule-membrane fusion and degranulation. [provided by RefSeq]
Product Categories/Family for anti-GCA antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Grancalcin, EF-hand calcium binding protein
NCBI Official Synonym Full Names
grancalcin
NCBI Official Symbol
GCA
NCBI Official Synonym Symbols
GCL
NCBI Protein Information
grancalcin
Protein Family
NCBI Description
This gene encodes a calcium-binding protein that is abundant in neutrophils and macrophages. In the absence of divalent cation, this protein localizes to the cytosolic fraction; with magnesium alone, it partitions with the granule fraction; and in the presence of magnesium and calcium, it associates with both the granule and membrane fractions. Alternative splicing and use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Aug 2016]
Research Articles on GCA
Similar Products
Product Notes
The GCA (Catalog #AAA6169469) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GCA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the GCA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GCA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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