Rabbit anti-Human EPCR Monoclonal Antibody | anti-PROCR antibody

Recombinant Anti-EPCR Antibody, Rabbit Monoclonal

Cat. Num. AAA8106889

• Reactivity: Human
• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein A
• Synonyms: EPCR; Monoclonal Antibody; Recombinant Anti-EPCR Antibody; Rabbit Monoclonal; Epcr/PROCR Antibody; Rabbit MAb; protein C receptor; endothelial; Anti-CCCA Antibody; Anti-CCD41 Antibody; Anti-EPCR Antibody; anti-PROCR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 041
• Specificity: Human Epcr/PROCR.; No cross-reactivity in ELISA with Mouse PROCR
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-PROCR antibody: Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: IHC-P: 1:100-1:500; ICC/IF: 1:20-1:100
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Human Epcr/PROCR protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human Epcr/PROCR (rh Epcr/PROCR).
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human PROCR in human liver with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human PROCR in human kidney with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).)

Immunofluorescence (IF)

(Immunofluorescence staining of PROCR in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Y PROCR monoclonal antibody (dilution ratio 1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm and cell membrane.)

Related Product Information for anti-PROCR antibody

Endothelial protein C receptor (EPCR), also known as activated protein C receptor (APC receptor) or PROCR, is a receptor for Protein C. Protein C plays an important role in many metabolism processes in humans and other animals after activated by binding to Endothelial protein C receptor (EPCR). Because of the EPCR is found primarily on endothelial cells (cells on the inside of blood vessels), activated protein C is found mainly near endothelial cells. Protein C is pleiotropic, with two main functions: anticoagulation and cytoprotection. Which function will be performed depending on whether or not protein C remains bind to EPCR after activated. The anticoagulation occurs when it does not. In this case, protein C functions as an anticoagulant by irreversibly proteolytically inactivating Factor Va and Factor VIIIa, turning them into Factor Vi and Factor VIIIi respectively. When still bound to EPCR, activated protein C performs its cytoprotective effects, acting on the effector substrate PAR-1, protease-activated receptor-1. To a degree, APC's anticoagulant properties are independent of its cytoprotective ones, in that expression of one pathway is not affected by the existence of the other.

Product Categories/Family for anti-PROCR antibody

Serine Proteases and Regulators

References

Nicolaes GA, et al. (2003). Congenital and acquired activated protein C resistance. Semin Vasc Med. 3 (1): 33-46.Esmon CT. (2003). The protein C pathway. Chest 124 (3): 26-32.Mosnier LO, et al. (2007)The cytoprotective protein C pathway. Blood. 109: 3161-72.

NCBI and Uniprot Product Information

• NCBI GI #: 48146013
• UniProt Accession #: Q9UNN8; Q14218; Q6IB56; Q96CB3; Q9ULX1; B2RC04
• Molecular Weight: 26,671 Da
• NCBI Official Full Name: PROCR
• UniProt Protein Name: Endothelial protein C receptor
• Protein Family: Endothelial protein C receptor
• UniProt Gene Name: PROCR
• UniProt Synonym Gene Names: EPCR; APC receptor
• UniProt Entry Name: EPCR_HUMAN

NCBI Description

The protein encoded by this gene is a receptor for activated protein C, a serine protease activated by and involved in the blood coagulation pathway. The encoded protein is an N-glycosylated type I membrane protein that enhances the activation of protein C. Mutations in this gene have been associated with venous thromboembolism and myocardial infarction, as well as with late fetal loss during pregnancy. The encoded protein may also play a role in malarial infection and has been associated with cancer. [provided by RefSeq, Jul 2013]

Uniprot Description

EPCR: a single-pass type I membrane protein. Binds activated protein C (APC), a serine protease that regulates thrombin (IIa) production. Plays a pivotal role in preventing blood coagulation through binding of activated protein C. Expressed strongly in the endothelial cells of arteries and veins in heart and lung, less intensely in capillaries in the lung and skin, and not at all in the endothelium of small vessels of the liver and kidney. The binding of APC to EPCR and PAR-1 increases invasion and chemotaxis.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 20q11.2

Cellular Component: centrosome; cell surface; focal adhesion; integral to plasma membrane; plasma membrane; extracellular region

Molecular Function: protein binding; receptor activity

Biological Process: antigen processing and presentation; immune response; blood coagulation; negative regulation of coagulation; leukocyte migration