Epcr / PROCR recombinant protein

Recombinant Human Epcr / PROCR Protein (His tag)

Cat. Num. AAA2545333

• Gene Names: PROCR; CCCA; EPCR; CCD41
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: Epcr / PROCR; Recombinant Human Epcr / PROCR Protein (His tag); CCCA; CCD41; CD201; EPCR; bA42O4.2; Epcr / PROCR recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 238

• Application Notes: The secreted recombinant human PROCR consists of 203 amino acids and has a predicted molecular mass of 23.4 kDa. The apparent molecular mass of rhPROCR is approximately 37 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Predicted N Terminal: Ser 18
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for Epcr / PROCR recombinant protein

Background: Endothelial protein C receptor (EPCR), also known as activated protein C receptor (APC receptor) or PROCR, is a receptor for Protein C. Protein C plays an important role in many metabolism processes in humans and other animals after activated by binding to Endothelial protein C receptor (EPCR). Because of the EPCR is found primarily on endothelial cells (cells on the inside of blood vessels), activated protein C is found maily near endothelial cells. Protein C is pleiotropic, with two main functions: anticoagulation and cytoprotection. Which function will be performed depend on whether or not protein C remains bind to EPCR after activated. The anticoagulation occurs when it does not. In this case, protein C functions as an anticoagulant by irreversibly proteolytically inactivating Factor Va and Factor VIIIa, turning them into Factor Vi and Factor VIIIi respectively. When still bound to EPCR, activated protein C performs its cytoprotective effects, acting on the effector substrate PAR-1, protease-activated receptor-1. To a degree, APC's anticoagulant properties are independent of its cytoprotective ones, in that expression of one pathway is not affected by the existence of the other. 

Description: A DNA sequence encoding the extracellular domain of human PROCR (Q9UNN8) (Met 1-Thr 209), was fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 34335272
• NCBI GeneID: 10544
• NCBI Accession #: NP_006395.2
• NCBI GenBank Nucleotide #: NM_006404.4
• UniProt Accession #: Q9UNN8; Q14218; Q6IB56; Q96CB3; Q9ULX1; B2RC04
• Molecular Weight: 26,671 Da
• NCBI Official Full Name: endothelial protein C receptor
• NCBI Official Synonym Full Names: protein C receptor
• NCBI Official Symbol: PROCR
• NCBI Official Synonym Symbols: CCCA; EPCR; CCD41
• NCBI Protein Information: endothelial protein C receptor
• UniProt Protein Name: Endothelial protein C receptor
• UniProt Gene Name: PROCR
• UniProt Synonym Gene Names: EPCR; APC receptor

NCBI Description

The protein encoded by this gene is a receptor for activated protein C, a serine protease activated by and involved in the blood coagulation pathway. The encoded protein is an N-glycosylated type I membrane protein that enhances the activation of protein C. Mutations in this gene have been associated with venous thromboembolism and myocardial infarction, as well as with late fetal loss during pregnancy. The encoded protein may also play a role in malarial infection and has been associated with cancer. [provided by RefSeq, Jul 2013]

Uniprot Description

EPCR: a single-pass type I membrane protein. Binds activated protein C (APC), a serine protease that regulates thrombin (IIa) production. Plays a pivotal role in preventing blood coagulation through binding of activated protein C. Expressed strongly in the endothelial cells of arteries and veins in heart and lung, less intensely in capillaries in the lung and skin, and not at all in the endothelium of small vessels of the liver and kidney. The binding of APC to EPCR and PAR-1 increases invasion and chemotaxis.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 20q11.22

Cellular Component: cell surface; extracellular region; focal adhesion; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; receptor activity

Biological Process: blood coagulation; leukocyte migration; negative regulation of coagulation