EPCR Recombinant Protein | PROCR recombinant protein

EPCR Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121375

• Purity: >97% as determined by SDS-PAGE
• Synonyms: EPCR; EPCR Protein; Human; Recombinant (His Tag); Human Epcr/PROCR Protein (His Tag); CCCA Protein; CCD41 Protein; protein C receptor; endothelial; PROCR recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Thr209

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ser 18
• Tag: C-His
• Protein Construction: A DNA sequence encoding the extracellular domain of human PROCR (Q9UNN8)(Met 1-Thr 209), was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PROCR recombinant protein

Background: Endothelial protein C receptor (EPCR), also known as activated protein C receptor (APC receptor) or PROCR, is a receptor for Protein C. Protein C plays an important role in many metabolism processes in humans and other animals after activated by binding to Endothelial protein C receptor (EPCR). Because of the EPCR is found primarily on endothelial cells (cells on the inside of blood vessels), activated protein C is found mainly near endothelial cells. Protein C is pleiotropic, with two main functions: anticoagulation and cytoprotection. Which function will be performed depending on whether or not protein C remains bind to EPCR after activated. The anticoagulation occurs when it does not. In this case, protein C functions as an anticoagulant by irreversibly proteolytically inactivating Factor Va and Factor VIIIa, turning them into Factor Vi and Factor VIIIi respectively. When still bound to EPCR, activated protein C performs its cytoprotective effects, acting on the effector substrate PAR-1, protease-activated receptor-1. To a degree, APC's anticoagulant properties are independent of its cytoprotective ones, in that expression of one pathway is not affected by the existence of the other.

Product Categories/Family for PROCR recombinant protein

Serine Proteases and Regulators

References

Nicolaes GA, et al. (2003). Congenital and acquired activated protein C resistance. Semin Vasc Med. 3 (1): 33-46.Esmon CT. (2003). The protein C pathway. Chest 124 (3): 26-32.Mosnier LO, et al. (2007)The cytoprotective protein C pathway. Blood. 109: 3161-72.

NCBI and Uniprot Product Information

• NCBI GI #: 48146013
• UniProt Accession #: Q9UNN8; Q14218; Q6IB56; Q96CB3; Q9ULX1; B2RC04
• Molecular Weight: 26,671 Da
• NCBI Official Full Name: PROCR
• UniProt Protein Name: Endothelial protein C receptor
• Protein Family: Endothelial protein C receptor
• UniProt Gene Name: PROCR
• UniProt Synonym Gene Names: EPCR; APC receptor
• UniProt Entry Name: EPCR_HUMAN

NCBI Description

The protein encoded by this gene is a receptor for activated protein C, a serine protease activated by and involved in the blood coagulation pathway. The encoded protein is an N-glycosylated type I membrane protein that enhances the activation of protein C. Mutations in this gene have been associated with venous thromboembolism and myocardial infarction, as well as with late fetal loss during pregnancy. The encoded protein may also play a role in malarial infection and has been associated with cancer. [provided by RefSeq, Jul 2013]

Uniprot Description

EPCR: a single-pass type I membrane protein. Binds activated protein C (APC), a serine protease that regulates thrombin (IIa) production. Plays a pivotal role in preventing blood coagulation through binding of activated protein C. Expressed strongly in the endothelial cells of arteries and veins in heart and lung, less intensely in capillaries in the lung and skin, and not at all in the endothelium of small vessels of the liver and kidney. The binding of APC to EPCR and PAR-1 increases invasion and chemotaxis.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 20q11.2

Cellular Component: centrosome; cell surface; focal adhesion; integral to plasma membrane; plasma membrane; extracellular region

Molecular Function: protein binding; receptor activity

Biological Process: antigen processing and presentation; immune response; blood coagulation; negative regulation of coagulation; leukocyte migration