Western Blot (WB)
(PSMB11 Antibody (C-term) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the PSMB11 antibody detected the PSMB11 protein (arrow).)
Rabbit anti-Human PSMB11 Polyclonal Antibody | anti-PSMB11 antibody
PSMB11 Antibody (C-term)
IHC-P~~1:50~100
Western Blot (WB)
(PSMB11 Antibody (C-term) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the PSMB11 antibody detected the PSMB11 protein (arrow).)
Western Blot (WB)
(PSMB11 Antibody (C-term) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the PSMB11 antibody detected the PSMB11 protein (arrow).)
Western Blot (WB)
(Anti-PSMB11 Antibody (C-term) at 1:1000 dilution + T47D whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 33 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-PSMB11 Antibody (C-term) at 1:1000 dilution + T47D whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 33 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Immunohistochemistry (IHC)
(PSMB11 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human thymoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PSMB11 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(PSMB11 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human thymoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PSMB11 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Murata, S., et al. Science 316(5829):1349-1353(2007)
NCBI and Uniprot Product Information
NCBI Description
Proteasomes generate peptides that are presented by major histocompatibility complex (MHC) I molecules to other cells of the immune system. Proteolysis is conducted by 20S proteasomes, complexes of 28 subunits arranged as a cylinder in 4 heteroheptameric rings: alpha-1 to -7, beta-1 to -7, beta-1 to -7, and alpha-1 to -7. The catalytic subunits are beta-1 (PSMB6; MIM 600307), beta-2 (PSMB7; MIM 604030), and beta-5 (PSMB5; MIM 600306). Three additional subunits, beta-1i (PSMB9; MIM 177045), beta-2i (PSMB10; MIM 176847), and beta-5i (PSMB8; MIM 177046), are induced by gamma-interferon (IFNG; MIM 147570) and are preferentially incorporated into proteasomes to make immunoproteasomes. PSMB11, or beta-5t, is a catalytic subunit expressed exclusively in cortical thymic epithelial cells (Murata et al., 2007 [PubMed 17540904]).[supplied by OMIM, Mar 2008]
Uniprot Description
PSMB11: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Incorporated instead of PSMB5 or PSMB8, this unit reduces the chymotrypsin-like activity of the proteasome. Plays a pivotal role in development of CD8-positive T cells. Belongs to the peptidase T1B family.
Protein type: Proteasome complex; EC 3.4.25.1; Protease
Chromosomal Location of Human Ortholog: 14q11.2
Cellular Component: proteasome core complex; nucleus; cytosol
Molecular Function: threonine endopeptidase activity
Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis
Research Articles on PSMB11
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Product Notes
The PSMB11 psmb11 (Catalog #AAA9205519) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PSMB11 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PSMB11 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-p). WB~~1:1000 IHC-P~~1:50~100. Researchers should empirically determine the suitability of the PSMB11 psmb11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PSMB11, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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