Western Blot (WB)
(Western Blot detection of EAPII with EAPII antibody in OV2008 human cancer cell line (b) and purified EAPII protein (a) as control. In cell lines, polyclonal often detects an additional higher molecular weight band that may represent a homo- or heterodimer.)
Rabbit anti-Human EAPII Polyclonal Antibody | anti-TDP2 antibody
EAPII (ETS1-Associated Protein 2)
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Western Blot (WB)
(Western Blot detection of EAPII with EAPII antibody in OV2008 human cancer cell line (b) and purified EAPII protein (a) as control. In cell lines, polyclonal often detects an additional higher molecular weight band that may represent a homo- or heterodimer.)
Western Blot (WB)
(Western Blot detection of EAPII with EAPII antibody in OV2008 human cancer cell line (b) and purified EAPII protein (a) as control. In cell lines, polyclonal often detects an additional higher molecular weight band that may represent a homo- or heterodimer.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of a superfamily of divalent cation-dependent phosphodiesterases. The encoded protein associates with CD40, tumor necrosis factor (TNF) receptor-75 and TNF receptor associated factors (TRAFs), and inhibits nuclear factor-kappa-B activation. This protein has sequence and structural similarities with APE1 endonuclease, which is involved in both DNA repair and the activation of transcription factors. [provided by RefSeq]
Uniprot Description
TTRAP: DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. Hydrolyzes 5'- phosphoglycolates on protruding 5' ends on DNA double-strand breaks (DSBs) due to DNA damage by radiation and free radicals. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Has preference for single-stranded DNA or duplex DNA with a 4 base pair overhang as substrate. Has also 3'- tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. Constitutes the major if not only 5'- tyrosyl-DNA phosphodiesterase in cells. Also acts as a 5'-tyrosyl- RNA phosphodiesterase following picornavirus infection: its activity is hijacked by picornavirus and acts by specifically cleaving the protein-RNA covalent linkage generated during the viral genomic RNA replication steps of a picornavirus infection, without impairing the integrity of viral RNA. Also acts as an adapter by participating to the specific activation of MAP3K7/TAK1 in response to TGF-beta: associates with components of the TGF- beta receptor-TRAF6-TAK1 signaling module and promotes their ubiquitination dependent complex formation. Involved in non- canonical TGF-beta induced signaling routes. May also act as a negative regulator of ETS1 and may inhibit NF-kappa-B activation. Acts as a regulator of ribosome biogenesis following stress. Belongs to the CCR4/nocturin family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Inhibitor; EC 3.1.4.-; Nucleolus
Chromosomal Location of Human Ortholog: 6p22.3-p22.1
Cellular Component: nucleoplasm; PML body; intercellular bridge; cytoplasm; nucleolus; nucleus
Molecular Function: protein binding; nuclease activity; manganese ion binding; magnesium ion binding; transcription corepressor activity; single-stranded DNA binding
Biological Process: cell surface receptor linked signal transduction; double-strand break repair
Research Articles on TDP2
2. TTRAP is the first human 5'-tyrosyl DNA phosphodiesterase to be identified, and we suggest that this enzyme is denoted tyrosyl DNA phosphodiesterase-2 (TDP2).
3. This is the first time that TTRAP has been shown to interact with HIV-1 integrase and facilitate lentiviral vector integration.
4. These results suggest an active role of DJ-1 missense mutants in the control of cell death and position TTRAP as a new player in the arena of neurodegeneration.
5. TTRAP is a novel PML nuclear body protein.
6. a significant association with (TTRAP)on reading and spelling ability was observed
7. interacts with ETS1 and modulates its transcriptional function
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Product Notes
The TDP2 tdp2 (Catalog #AAA617714) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EAPII (ETS1-Associated Protein 2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EAPII can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Western Blot: Use at 1:1000 to 1:5000. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry. Researchers should empirically determine the suitability of the TDP2 tdp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EAPII, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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