Mouse anti-Human DOC2A Monoclonal Antibody | anti-DOC2A antibody

DOC2A (Double C2-like Domain-containing Protein alpha, Doc2, Doc2-alpha)

Cat. Num. AAA6009807

• Gene Names: DOC2A; Doc2
• Reactivity: Human
• Applications: ELISA
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: DOC2A; Monoclonal Antibody; DOC2A (Double C2-like Domain-containing Protein alpha; Doc2; Doc2-alpha); Anti -DOC2A (Double C2-like Domain-containing Protein alpha; anti-DOC2A antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 3C11
• Specificity: Recognizes human DOC2A.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MRGRRGDRMTINIQEHMAINVCPGPIRPIRQISDYFPRGPGPEGGGGSGGEAPAHLVPLALAPPAALLGATTPEDGAEVDSYDSDDATALGTLEFDLLYDRASCTLHCSILRAKGLKPMDFNGLADPYVKLHLLPGACKANKLKTKTQRNTLNPVWNEDLTYSGITDDDITHKVLRIAVCDEDKLSHNEFIGEIRVPLRRLKPSQKKHFNICLERQVPLASPSSMSAALRGISCYLKELEQAEQGQGLLEERGRI

• Applicable Applications for anti-DOC2A antibody: ELISA (EL/EIA)
• Application Notes: Suitable for use in ELISA.
• Immunogen: Full length recombinant corresponding to aa1-401 from DOC2A (AAH63436) with GST tag. MW of the GST tag alone is 26kD.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Detection limit for recombinant GST tagged DOC2A is 1ng/ml as a capture antibody.)

Related Product Information for anti-DOC2A antibody

Doc2-alpha is mainly expressed in brain and is suggested to be involved in Ca(2+)-dependent neurotransmitter release. It may also be involved in dynein-dependent intracellular vesicle transport. The cDNA predicts a 400-amino acid protein with relative mass of 44,071 Da and 2 repeated C2-like domains in the C-terminal region. The C2 domain 1 is involved in binding calcium and phospholipids. It is predominantly expressed in the brain but also expressed in testis.

Product Categories/Family for anti-DOC2A antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 530719517
• NCBI GeneID: 8448
• NCBI Accession #: NP_001268997.1
• NCBI GenBank Nucleotide #: NM_001282068.1
• UniProt Accession #: Q14183; Q6P4G4; Q7Z5G0; Q8IVX0
• Molecular Weight: 43,959 Da
• NCBI Official Full Name: double C2-like domain-containing protein alpha
• NCBI Official Synonym Full Names: double C2-like domains, alpha
• NCBI Official Symbol: DOC2A
• NCBI Official Synonym Symbols: Doc2
• NCBI Protein Information: double C2-like domain-containing protein alpha; doc2-alpha
• UniProt Protein Name: Double C2-like domain-containing protein alpha
• Protein Family: Double C2-like domain-containing protein
• UniProt Gene Name: DOC2A
• UniProt Synonym Gene Names: Doc2; Doc2-alpha
• UniProt Entry Name: DOC2A_HUMAN

NCBI Description

There are at least two protein isoforms of the Double C2 protein, namely alpha (DOC2A) and beta (DOC2B), which contain two C2-like domains. DOC2A and DOC2B are encoded by different genes; these genes are at times confused with the unrelated DAB2 gene which was initially named DOC-2. DOC2A is mainly expressed in brain and is suggested to be involved in Ca(2+)-dependent neurotransmitter release. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

DOC2A: Calcium sensor which most probably regulates fusion of vesicles with membranes. Binds calcium and phospholipids. May be involved in calcium dependent neurotransmitter release through the interaction with UNC13A. May be involved in calcium-dependent spontaneous release of neurotransmitter in absence of action potentials in neuronal cells. Regulates Ca(2+)-dependent secretory lysosome exocytosis in mast cells.

Protein type: Vesicle; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: neuron projection; synaptic vesicle membrane; lysosome; cell junction

Molecular Function: protein binding; calcium-dependent phospholipid binding; transporter activity

Biological Process: synaptic transmission; nervous system development; synaptic vesicle exocytosis; regulation of calcium ion-dependent exocytosis