Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Thyroglobulin (TG) Standard Curve.)
Rat Thyroglobulin (TG) ELISA Kit | TG elisa kit
Rat Thyroglobulin (TG) ELISA Kit
Gene Names
Tg; Tgn
Reactivity
Rat
Synonyms
Thyroglobulin (TG); Rat Thyroglobulin (TG) ELISA Kit; TG; AITD3; TGN; TG elisa kit
Reactivity
Rat
Specificity
Rat Thyroglobulin (TG) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TG. No significant cross-reactivity or interference between Rat TG and analogues was observed.
Sequence Length
2768
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Thyroglobulin (TG) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Thyroglobulin (TG) Standard Curve.)
Related Product Information for TG elisa kit
Description: This Rat Thyroglobulin (TG) ELISA Kit employs a two-site sandwich ELISA to quantitate TG in samples. An antibody specific for TG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Thyroglobulin (Tg) is a glycoprotein homodimer produced predominantly by the thryroid gland. It acts as a substrate for the synthesis of thyroxine and triiodothyronine as well as the storage of the inactive forms of thyroid hormone and iodine. Thyroglobulin is secreted from the endoplasmic reticulum to its site of iodination, and subsequent thyroxine biosynthesis, in the follicular lumen. Mutations in this gene cause thyroid dyshormonogenesis, manifested as goiter, and are associated with moderate to severe congenital hypothyroidism. Polymorphisms in this gene are associated with susceptibility to autoimmune thyroid diseases (AITD) such as Graves disease and Hashimoto thryoiditis. [provided by RefSeq, Nov 2009]
Background: Thyroglobulin (Tg) is a glycoprotein homodimer produced predominantly by the thryroid gland. It acts as a substrate for the synthesis of thyroxine and triiodothyronine as well as the storage of the inactive forms of thyroid hormone and iodine. Thyroglobulin is secreted from the endoplasmic reticulum to its site of iodination, and subsequent thyroxine biosynthesis, in the follicular lumen. Mutations in this gene cause thyroid dyshormonogenesis, manifested as goiter, and are associated with moderate to severe congenital hypothyroidism. Polymorphisms in this gene are associated with susceptibility to autoimmune thyroid diseases (AITD) such as Graves disease and Hashimoto thryoiditis. [provided by RefSeq, Nov 2009]
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Thyroglobulin
NCBI Official Synonym Full Names
thyroglobulin
NCBI Official Symbol
Tg
NCBI Official Synonym Symbols
Tgn
NCBI Protein Information
thyroglobulin
UniProt Protein Name
Thyroglobulin
Protein Family
UniProt Gene Name
Tg
UniProt Synonym Gene Names
Tg
NCBI Description
precursor to thyroid hormone; required for thyroid hormone generation [RGD, Feb 2006]
Uniprot Description
Precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3).
Research Articles on TG
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Product Notes
The Rat TG tg (Catalog #AAA9711819) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711819 ELISA Kit recognizes Rat TG. It is sometimes possible for the material contained within the vial of "Thyroglobulin (TG), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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